Key facts
- Brand
- Agilent
- Model
- DB-5MS
- Industry
- Industrial
- Documents
- 5
- Languages
- EN
- Last added
- 2012-06-12
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Agilent Trace-Level Analysis of Melamine in Milk Products on Agilent 7890A/5975C GC/MSD Using a New Agilent J W DB-5ms Ultra Inert Column SampliQ SCX Cartridges Manual
The document presents a Gas Chromatography/Mass Spectrometry (GC/MS) method for the quantitative determination and confirmation of melamine residues in milk products. The sample preparation involves cleaning up milk samples using Agilent's new SampliQ SCX SPE cartridges followed by derivatization.
The derived extracts are analyzed by GC/MS on a new Agilent J&W DB-5ms Ultra Inert column operating in synchronous SIM/scan mode. The method demonstrates good linearity, high recoveries over 80 percent, and a low limit of detection (LOD) of 0.0025 mg/kg suitable for trace-level analysis.
Agilent DB-5MS specifications
| Column Length | 20 m |
|---|---|
| Column ID | 0.18 mm |
| Part Number (DB-EUPAH) | 121-9627 |
| Part Number (DB-5ms) | 121-5522 |
| Analysis Time (DB-EUPAH) | Under 28 minutes |
Source: Agilent DB-EUPAH and DB-5ms High Efficiency GC Column Selection Guide
All Agilent DB-5MS documents by type
How to use the product for one specific task, with worked examples.
- Agilent Trace-Level Analysis of Melamine in Milk Products on Agilent 7890A/5975C GC/MSD Using a New Agilent J W DB-5ms Ultra Inert Column SampliQ SCX Cartridges Manual 6 p
- Agilent J&W DB-5ms Ultra Inert Capillary GC Column Application Brief for PAH Analysis 2 p
- Agilent J&W DB-5ms Ultra Inert GC Column PBDE Analysis Application Note 2 p
- Agilent DB-5ms Ultra Inert GC Column PAH Analysis Application Note 8 p
Questions answered in these manuals
What is the lowest concentration of melamine this method can detect in milk?
The limit of detection for melamine in the milk matrix is 0.0025 mg/kg.
What is the linear concentration range for this melamine analysis method?
The method shows good linearity in the range of 0.025 to 2.000 pg/mL.
Which column is recommended for separating benzo[b,j,k]fluoranthene isomers?
The Agilent J&W DB-EUPAH column is recommended when separation of benzo[b,j,k]fluoranthene isomers is required.
From: Agilent DB-EUPAH and DB-5ms High Efficiency GC Column Selection Guide
How long does the analysis take when using the DB-EUPAH column?
The separation of all 24 regulated PAHs is achieved in under 28 minutes.
From: Agilent DB-EUPAH and DB-5ms High Efficiency GC Column Selection Guide
How long is the analysis time for the PAH mixture?
Good resolution was obtained in a 15-min analysis for each of the PAHs.
From: Agilent J&W DB-5ms Ultra Inert Capillary GC Column Application Brief for PAH Analysis
What is the minimum concentration range demonstrated for PAH analysis?
The application demonstrates trace-level analyses over a concentration range of 0.05 pg/mL to 5 pg/mL.
From: Agilent J&W DB-5ms Ultra Inert Capillary GC Column Application Brief for PAH Analysis