Agilent DB-5MS Manuals

A GC/MS method is presented for the quantitative determination and confirmation of melamine residues in milk products.

This document describes the results of trace-level PAH analyses using Agilent J&W DB-5ms Ultra Inert 30 m × 0.25 mm, 0.25 µm capillary GC column on a 16-component PAH standard mixture.

This application demonstrates the use of electron impact GC–MS for trace-level PBDE analysis. The method uses Agilent J&W DB-5ms Ultra Inert GC column. This column has been thoroughly tested for column inertness and provides the analyst with high confidence in the accuracy of the results.

This application note details a quick and effective analytical method for the determination of low and trace level polycyclic aromatic hydrocarbons (PAHs) in fish samples as an alternative to procedures involving more time consuming and complex techniques. A simplified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method with dispersive solid phase extraction (dSPE) provided sufficient sample matrix cleanup while preserving low level analyte detection. A pressure controlled tee was installed post column to implement the use of automated backflush to diminish residual sample carryover and reduce instrument cycle times. The Agilent J&W DB-5ms Ultra Inert (UI) 20 m × 0.18 mm , 0.18 µm column effectively resolved the 16 targeted PAHs. A GC/MS method employing selective ion monitoring (SIM) was calibrated at the 10, 25, 50, 100, 250, 500, and 1000 ng/mL PAH levels, yielding excellent linearity and reproducibility. The spiked levels for recovery determinations were 25, 250, and 500 ng/mL. Recoveries ranged from 80% and 139% with RSD below 6%.