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Agilent Analysis of Amitriptyline, Fluoxetine, Quetiapine, and Sertraline Using Dried Blood Spots Application Note
This document details a method for analyzing amitriptyline, fluoxetine, quetiapine, and sertraline using dried blood spots (DBS) and a new non-cellulose Dried Matrix Spotting (DMS) media. The procedure involves spotting 15 microliters of blood onto a card, drying it, punching a 2-4 mm core, and desorbing the analytes with solvent for LC-MS/MS analysis.
Experimental results demonstrate the method's performance using an Agilent 1290 LC system coupled with an Agilent 6460 mass spectrometer. The study achieved linearity with correlation coefficients better than 0.995 and relative recoveries within 10% of the true value across three concentration levels (0.5, 5.0, and 500 ng/mL).
Agilent 1290 LC specifications
| Column | Agilent Poroshell 120 EC-C18 3.0 mm x 50 mm |
|---|---|
| Mobile Phase A | 0.1% Aqueous formic acid |
| Runtime | 3:00 minutes |
| Gas temp | 350 C |
| Gas flow | 10 L/min |
| Nebulizing | 20 psi |
All Agilent 1290 LC documents by type
How to use the product for one specific task, with worked examples.
- Agilent Analysis of Amitriptyline, Fluoxetine, Quetiapine, and Sertraline Using Dried Blood Spots Application Note 4 p
- Agilent Analysis of Clozapine Nortriptyline Paroxetine Zolpidem Using Dried Blood Spots Application Note 4 p
- Agilent 1260/1290 LC Analysis of Bisphenol A Leaching from Baby Feeding Bottles Application Note 12 p
Questions answered in these manuals
What system was used for the analysis?
The analysis was performed using an Agilent 1290 LC system and an Agilent 6460 mass spectrometer.
What is the recommended drying time for blood spots?
The blood spot should be allowed to dry for at least 2 hours.
What analytes were analyzed in this study?
The study analyzed Clozapine, Nortriptyline, Paroxetine, and Zolpidem.
What equipment was used for the analysis?
The analysis was performed using an Agilent 1290 LC and 6460 Mass Spectrometer.
What is the limit of quantitation for bisphenol A?
The limit of quantitation (LOQ) for bisphenol A was found to be 1.06 ng/mL.
From: Agilent 1260/1290 LC Analysis of Bisphenol A Leaching from Baby Feeding Bottles Application Note
What was the sample recovery rate for bisphenol A?
During sample recovery studies, 80% recovery was obtained for bisphenol A.
From: Agilent 1260/1290 LC Analysis of Bisphenol A Leaching from Baby Feeding Bottles Application Note