Key facts
- Brand
- Agilent
- Model
- G6410
- Documents
- 3
- Languages
- EN
- Last added
- 2012-06-12
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Agilent G6410 Rapid Sensitive Analysis Method for Sudan Reds in Curry Chili Powder Using LC/MS/MS Application Note
The document presents an analytical method developed for detecting Sudan Red I, II, III, IV, and Para Red in various matrices such as curry powder, red pepper powder, and Worcestershire sauce. The method utilizes the Agilent G6410 Triple Quadrupole LC/MS system with electrospray ionization (ESI) operated in positive mode to meet EU testing requirements for these prohibited food colorants.
The procedure details sample preparation techniques involving extraction with ethyl acetate, ultrasonication, and filtration. It specifies the chromatographic conditions using a ZORBAX XDB C18 column and provides specific Mass Spectrometry settings, including MRM transitions, fragmentor voltages, and collision energies optimized for each analyte to ensure high sensitivity and selectivity.
Agilent G6410 specifications
| Column | ZORBAX XDB C18 2.1 mm x 100 mm, 1.8 ym |
|---|---|
| Flow rate | 0.4 mL/min |
| Drying gas temp. | 350°C |
| Veap voltage | 3500 V |
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How to use the product for one specific task, with worked examples.
Questions answered in these manuals
Which Sudan Red dyes does this method analyze?
The method analyzes Para Red, Sudan Red I, Sudan Red II, Sudan Red III, and Sudan Red IV.
What is the detection limit demonstrated for this method?
The methodology demonstrates sensitivity with a spike of chili powder at 1 ppb.
What is the minimum detection limit for chloramphenicol in honey, shrimp, and chicken using this method?
The method provides a limit of detection at 10 ppt in the sample matrix.
From: Agilent G6410AA LC/MS Application Note: Chloramphenicol Analysis in Food
What is the maximum flow rate used for the HPLC system in this analysis?
The flow rate for the HPLC system is set to 0.4 mL/min.
From: Agilent G6410AA LC/MS Application Note: Chloramphenicol Analysis in Food
How many pesticides can be determined using this method?
The method is designed for the determination of 44 pesticide residues in several foodstuffs.
From: Agilent G6410AA Triple Quadrupole Mass Spectrometer Pesticide Determination Application Note
What is the limit of detection for the pesticides analyzed?
The limits of detection for all pesticides were less than 10 ng/mL in foodstuff.
From: Agilent G6410AA Triple Quadrupole Mass Spectrometer Pesticide Determination Application Note