Key facts
- Brand
- Agilent
- Model
- 6410A
- Documents
- 8
- Languages
- JA · EN
- Last added
- 2012-07-06
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Agilent 6410A Triple Quadrupole Mass Spectrometer Application Note
This document details an analytical method developed using the Agilent 6410A Triple Quadrupole Mass Spectrometer (QQQ) to screen and confirm 65 pharmaceuticals in water samples, following EPA Method 1694 guidelines. The method utilizes four different chromatographic gradients and LC conditions based on the polarity and class of the pharmaceuticals, employing both positive and negative ion electrospray ionization (ESI). This manual is written in Japanese.
Read onlineAgilent 6410A specifications
| Instrument Model | Agilent 6410A Triple Quadrupole Mass Spectrometer |
|---|---|
| Ionization Mode | Positive and Negative ESI |
| Nebulizer Pressure | 40 psig |
| Capillary Voltage | 4000V |
| Drying Gas Temperature | 300 C |
| Fragmenter Voltage | 70-130V |
| Collision Energy | 5-35 V |
| Dwell Time | 10 msec |
Source: Agilent 6410A Triple Quadrupole Mass Spectrometer Application Note
All Agilent 6410A documents by type
How to use the product for one specific task, with worked examples.
- Agilent 6410A Triple Quadrupole Mass Spectrometer Application Note 12 p
- Agilent EPA Method 1694: Agilent's 6410A LC/MS/MS Solution for Pharmaceuticals Personal Care Products in Water Soil Sediment Biosolids by HPLC/MS/MS Application Note 12 p
- Agilent 6410A LC/MS/MS Application Brief for Sulfonamide Analysis 4 p
- Agilent 6410A Triple Quadrupole LC/MS Application Brief for Chloramphenicol Analysis 4 p
- Agilent Development of an LC-MS/MS Method for the Determination of 20-Hydroxyecdysone Its Metabolites in Calf Urine Manual 6 p
- Agilent G6410AA LC/MS/MS Aflatoxin Determination Application Note 8 p
- Agilent G6410AA LC/MS Application Note: Chloramphenicol Analysis in Food 8 p
- Agilent G6410AA Triple Quadrupole Mass Spectrometer Pesticide Determination Application Note 12 p
Questions answered in these manuals
Which EPA method is this analysis based on?
The analysis follows the guidelines of EPA Method 1694.
From: Agilent 6410A Triple Quadrupole Mass Spectrometer Application Note
How many MRM transitions are used for each compound?
Two MRM transitions are used for each compound for quantification and qualitative ion analysis.
From: Agilent 6410A Triple Quadrupole Mass Spectrometer Application Note
How many analytes are included in this method?
The method includes 65 analytes, which is a mixture of PPCPs analyzed by a single MRM transition, plus 17 labeled internal standards.
What ionization modes are used?
The method uses positive and negative ion electrospray ionization (ESI+ and ESI-), depending on the specific analyte group.
What is the maximum residue level for sulfonamides in food-producing species according to EU regulations?
The existing EU maximum residue level for all drugs of the sulfonamide group is 100 pg/kg in all food-producing species.
From: Agilent 6410A LC/MS/MS Application Brief for Sulfonamide Analysis
How long does the sample homogenization step take in the described procedure?
The samples were homogenized for 3 minutes with 10 mL acidified methanol.
From: Agilent 6410A LC/MS/MS Application Brief for Sulfonamide Analysis