Key facts
- Brand
- Agilent
- Model
- 1200 Series RRLC AS
- Documents
- 2
- Languages
- EN
- Last added
- 2012-06-12
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Agilent 1200 Series RRLC AS Purification of PNA Application Note
This document describes an optimized HPLC method using the Agilent 1200 Series RRLC AS purification system to separate full-length peptide nucleic acids (PNAs) from truncated sequences and byproducts. The note details specific LC methods for both Fmoc-protected PNAs and dye-labeled PNAs, utilizing Agilent ZORBAX Eclipse columns.
Read onlineAgilent 1200 Series RRLC AS specifications
| Sample temperature | 4 °C |
|---|---|
| Column temperature | 45 °C |
| Flow rate (10 min method) | 1 mL/min |
| Flow rate (4 min method) | 2 mL/min |
| Purification time (optimized) | 4 minutes |
Source: Agilent 1200 Series RRLC AS Peptide Purification Application Note
All Agilent 1200 Series RRLC AS documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the purpose of this application note?
It demonstrates how to configure and use the Agilent 1200 Series RRLC AS purification system for peptide nucleic acids.
From: Agilent 1200 Series RRLC AS Purification of PNA Application Note
What is the flow rate for purifying Fmoc-PNA and dye-PNA?
The flow rate is set to 2 mL/min.
From: Agilent 1200 Series RRLC AS Purification of PNA Application Note
What system is used for peptide purification in this note?
The Agilent 1200 Series Rapid Resolution LC (RRLC) analytical scale (AS) purification system.
From: Agilent 1200 Series RRLC AS Peptide Purification Application Note
What is the optimized purification time for a 0.1-pmol synthesis scale?
The optimized method allows for purification within 4 minutes per peptide.
From: Agilent 1200 Series RRLC AS Peptide Purification Application Note