Key facts
- Brand
- Agilent
- Model
- 1220 Infinity
- Documents
- 6
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Time- peak-based fraction collection with the Agilent 1220 Infinity LC System Manual
This document provides a technical demonstration of analytical-scale purification using the Agilent 1260 Infinity Analytical-scale Fraction Collector. It details how to perform time- and peak-based fraction collection when combined with an Agilent 1220 Infinity LC System.
The text explains two critical prerequisites for high-purity preparative liquid chromatography: accurate delay time determination using the built-in Delay Sensor, and minimized system dispersion via optimized capillaries. It presents experimental results comparing time-based versus peak-based collection methods to illustrate purity recovery.
Agilent 1220 Infinity specifications
| Supported Software Versions | Empower 2, feature release 3 or higher or Empower 3 software |
|---|---|
| Operating System Requirement | Windows XP with service pack 3 or higher |
| Required Firmware Version | A.06.32 or B.06.32 or B.06.41 or higher |
| Required Driver Type | RC.Net drivers |
All Agilent 1220 Infinity documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the delay volume determined for the system setup described in this document?
The delay volume was determined to be 83 µL.
From: Agilent Time- peak-based fraction collection with the Agilent 1220 Infinity LC System Manual
Which Agilent 1220 Infinity LC configurations are supported by Empower?
Only systems containing an autosampler are supported; instruments with a manual injector are not.
What is the precision of retention times for six consecutive runs?
The precision for the retention times is less than 0.24% RSD.
What is the maximum pressure rating of the dual gradient pump?
The dual gradient pump has a maximum pressure rating of 600 bar.
What is the maximum flow rate supported by this system?
The system supports flow rates up to 10 mL/ min.
What is the detection limit for amoxicillin impurities?
Impurities down to a level of 0.01% could be detected under the specified conditions.
From: Agilent 1220 Infinity LC System Amoxicillin Impurity Analysis Application Note