Key facts
- Brand
- Agilent
- Model
- 1290 Infinity LC System
- Documents
- 28
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Increasing Resolution Speed by Operating UHPLC Columns up to 1200 Bar Technical Note Manual
This document explains that Ultra High Performance Liquid Chromatography (UHPLC) using sub-2-micron particles offers productivity benefits, but traditional systems are limited by backpressure caps of 400 to 600 bar. Agilent addresses this with the 1290 Infinity LC System and ZORBAX Rapid Resolution High Definition (RRHD) columns designed for operation up to 1200 bar.
The text details three examples demonstrating these capabilities: maximizing speed by running a gradient separation at 1.6 mL/min generating over 1070 bar; increasing resolution by using tandem columns in series requiring nearly 1100 bar; and improving peak capacity with longer columns operating around 800 bar.
Agilent 1290 Infinity LC System specifications
| Analysis time | 2 min |
|---|---|
| Backpressure | 1120 bar |
| Column upper limit | 1200 bar |
| Detection limit | 1 mg/kg |
| Column type | ZORBAX RRHD Eclipse Plus C18 |
| Column dimensions | 50 mm L x 2.1 mm id, 1.8 um d |
| Mobile phase A | 0.02% phosphoric acid in water |
| Mobile phase B | Acetonitrile/methanol 50/50 or 75/25 v/v |
Source: Agilent 1290 Infinity LC Application Note for Ultrafast Antioxidant Analysis
All Agilent 1290 Infinity LC System documents by type
How to use the product for one specific task, with worked examples.
- Agilent 1290 Infinity LC System Application Note for Screening Impurities in Fine Chemicals 10 p
- Agilent 1290 Infinity LC System Application Note for Cytostatic Drugs Analysis 8 p
- Agilent 1290 Infinity LC Application Note for Ultrafast Antioxidant Analysis 8 p
- Agilent 1290 Infinity LC System Analysis of TCM Injections Application Note 8 p
- Agilent 1290 Infinity LC Application Note for Sweetener Analysis 8 p
- Agilent 1290 Infinity LC Application Note for Aldehyde and Ketone Determination 4 p
- Agilent 1290 Infinity LC and 6460A MS Application Note for PGI Analysis 12 p
- Agilent Quantification of Alprazolam in Dried Blood Spots Using Agilent 1290 Infinity LC System and 6460 Triple Quadrupo 2 p
- Agilent Speed up the LC Analysis of Notoginsenoside R1 Ginsenosides Rg1 Re Rb1 in Compound TCM Using the Agilent 1290 Infinity LC system ZORBAX RRHD 1.8 m Column Manual 2 p
- Agilent 1290 Infinity LC Rapid Gradient Elevated Temperature UHPLC of Flavonoids Application Note 8 p
- Agilent 1290 Infinity LC System Application Note for Amino Acid Analysis 4 p
- Agilent 1290 Infinity LC System PAH Method Development Application Note 1 p
- Agilent 1290 Infinity LC System Application Note 8 p
- Maximizing Chromatographic Peak Capacity with the Agilent 1290 Infinity LC System Application Note 8 p
- Agilent 1290 Infinity LC Peak Capacity Application Note 8 p
- Agilent 1290 Infinity LC System Cleaning Validation Application Note Part 3 4 p
- Agilent 1290 Infinity LC System Application Note for Genotoxic Impurity Determination 8 p
- Agilent 1290 Infinity LC Application Note: Fast Analysis of Resveratrol in Red Wine 2 p
- Agilent 1290 Infinity LC Application Note: Determination of Explosives 12 p
- Agilent 1290 Infinity LC Application Note: High Sensitivity UHPLC-DAD Analysis of Azo Dyes 8 p
- Agilent 1290 Infinity LC and 6460 LC/MS Pesticide Determination Application Note 12 p
- Agilent 1290 Infinity LC System Application Note for Primary Aromatic Amine Detection 4 p
- Agilent Optimizing Performance of an Agilent ZORBAX RRHD Eclipse Plus C18 Column by Enhancing an Agilent 1290 Infinity LC System for Ultra-Low Dispersion Manual 8 p
- Agilent Increasing productivity in the analysis of impurities in metoclopramide hydrochloride formulations using the Agilent 1290 Infinity LC system
- Agilent Pesticide Analysis using an Agilent 1290 Infinity LC System with an Agilent 6140 Single Quadrupole LC/MS System Manual
- Agilent Profiling capsaicinoids in spicy foods food products using the Agilent 1290 Infinity LC system Agilent ZORBAX Poroshell 120 2.7 m columns with UV/Vis diode array fluorescence d
- Agilent Trace analysis of explosives in soil samples using the Agilent 1290 Infinity LC System equipped with an Agilent Max-Light 60-mm cartridge cell Manual
Questions answered in these manuals
What is the maximum operating pressure for Agilent ZORBAX RRHD columns?
These columns are designed for reliable operation at 1200 bar, which is the highest pressure in the industry.
From: Agilent Increasing Resolution Speed by Operating UHPLC Columns up to 1200 Bar Technical Note Manual
What is the maximum pressure reached during the antioxidant separation example?
The gradient program at a flow rate of 1.6 mL/min generated a maximum pressure of 1070 bar.
From: Agilent Increasing Resolution Speed by Operating UHPLC Columns up to 1200 Bar Technical Note Manual
What is the primary purpose of this application note?
The document demonstrates how to use the Agilent 1290 Infinity LC System with UV/VIS Diode Array detection to analyze octyl-dimethyl-p-aminobenzoic acid for impurities.
From: Agilent 1290 Infinity LC System Application Note for Screening Impurities in Fine Chemicals
What are the specific dimensions of the columns tested in this application?
The system was used with 3.0 and 2.1 mm id C18 columns, including a 3 mm x 50 mm column and a 2.1 mm x 150 mm column.
From: Agilent 1290 Infinity LC System Application Note for Screening Impurities in Fine Chemicals
What is the maximum pressure stability of the Agilent 1290 Infinity LC system?
The system has a pressure stability of up to 1200 bar.
From: Agilent 1290 Infinity LC System Application Note for Cytostatic Drugs Analysis
How much does the Agilent 1290 Infinity LC system reduce organic solvent consumption compared to the 1100 Series?
Using the 1290 Infinity LC system with a 2 mm id column reduces organic solvent consumption by 62 %.
From: Agilent 1290 Infinity LC System Application Note for Cytostatic Drugs Analysis