This application demonstrates the use of electron impact GC–MS for trace-level PBDE analysis. The method uses Agilent J&W DB-5ms Ultra Inert GC column. This column has been thoroughly tested for column inertness and provides the analyst with high confidence in the accuracy of the results.
This document describes a method for analyzing polybrominated diphenyl ethers (PBDEs) using electron impact gas chromatography-mass spectrometry (GC-MS). The application highlights the use of the Agilent J&W DB-5ms Ultra Inert capillary GC column to achieve successful separation of challenging analytes, particularly BDE-209, by minimizing column activity and residence time at high temperatures.
The study details the chromatographic conditions, including the use of a 15-meter column length to limit thermal exposure and a specific temperature program. Results indicate excellent linearity with R² values of 0.997 or greater and a signal-to-noise ratio greater than three to one for BDE-209 at a loading of 0.025 ng.
| Column Length | 15 m |
|---|---|
| Column Dimensions | 15 m x 0.25 mm, 0.25 µm |
| Column Part Number | 122-5512UI |
| Carrier Gas | Helium |
| Inlet Temperature | 325 °C |
| Oven Temperature Range | 150 to 325 °C |
| Oven Ramp Rate | 17 °C/min |
| Oven Hold Time | 5 min |
| MSD Source Temperature | 300 °C |
| Quadrupole Temperature | 150 °C |
| Transfer Line Temperature | 300 °C |
| Scan Range | 200-1000 amu |
| Linearity R² | 0.997 or greater |
A 15-meter long column was used to limit residence time for BDE-209.
The signal-to-noise ratio was greater than three to one.
The part number is 122-5512UI.