Agilent Trace-Level Analysis of Melamine in Milk Products on Agilent 7890A/5975C GC/MSD Using a New Agilent J W DB-5ms Ultra Inert Column SampliQ SCX Cartridges Manual

About this manual

The document presents a Gas Chromatography/Mass Spectrometry (GC/MS) method for the quantitative determination and confirmation of melamine residues in milk products. The sample preparation involves cleaning up milk samples using Agilent's new SampliQ SCX SPE cartridges followed by derivatization.

The derived extracts are analyzed by GC/MS on a new Agilent J&W DB-5ms Ultra Inert column operating in synchronous SIM/scan mode. The method demonstrates good linearity, high recoveries over 80 percent, and a low limit of detection (LOD) of 0.0025 mg/kg suitable for trace-level analysis.

What's inside

• Introduction
• Standards and Reagents
• Instruments
• Sample Preparation
• GC/MS Analysis
• Linearity and Recovery

Frequently asked questions

What is the lowest concentration of melamine this method can detect in milk?

The limit of detection for melamine in the milk matrix is 0.0025 mg/kg.

What is the linear concentration range for this melamine analysis method?

The method shows good linearity in the range of 0.025 to 2.000 pg/mL.