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Agilent SepTech ST150 10-C18 Peptide Resolution Application Note
Agilent Technologies investigated alternative solvents for reversed phase HPLC analysis of peptides due to global shortages in acetonitrile. This document evaluates ethanol as a viable substitute, comparing its performance against acetonitrile and three leading competitor columns.
The study utilizes the SepTech ST150 10-C18 column with a mixture of four peptides (Oxytocin, Angiotensin II, Angiotensin I, and Insulin). Results indicate that while ethanol provides similar solvent strength to acetonitrile, it alters selectivity, causing partial co-elution between insulin and angiotensin I. Additionally, ethanol exhibits higher UV absorbance at 220 nm compared to acetonitrile.
Agilent SepTech ST150 10-C18 specifications
| Nominal Pore Size (ST60) | 60 A |
|---|---|
| Carbon Load (ST60) | 25 % |
| Operating pH Range | 1.5-10 |
All Agilent SepTech ST150 10-C18 documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the stated particle size of the SepTech ST150 10-C18 column?
The column has a particle size of 10 micrometers.
From: Agilent SepTech ST150 10-C18 Peptide Resolution Application Note
What is the recommended flow rate for the peptide separation described?
The procedure specifies a flow rate of 1.0 mL/min.
From: Agilent SepTech ST150 10-C18 Peptide Resolution Application Note
Which pore size is better for peptide separations?
The larger pore size material, SepTech ST150 10-C18, is required for viable peptide separations because the 60A pore size is too small for efficient diffusion.
How does pore size affect resolution?
Resolution is improved by using the larger pore size material, as it allows better access to the internal surface area for larger solutes.