Key facts
- Brand
- Agilent
- Model
- DE3
- Industry
- Industrial
- Documents
- 2
- Languages
- EN
- Last added
- 2012-06-06
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Agilent Technologies BL21-Gold Competent Cells BL21-Gold(DE3) Competent Cells BL21-Gold(DE3)pLysS Competent Cells Instruction Manual
The document is an instruction manual for three specific strains of E. coli competent cells: BL21-Gold, BL21-Gold(DE3), and BL21-Gold(DE3)pLysS. These high-performance cells are designed for protein expression studies utilizing the T7 RNA polymerase promoter. They feature improved transformation efficiency (greater than 1 x 10^8 cfu/ug of pUC18 DNA) and lack specific proteases like Lon and OmpT to prevent recombinant protein degradation.
The manual details the materials provided with each catalog number, storage conditions requiring -80°C, and specific transformation guidelines. It outlines protocols for transforming cells using heat pulses, inducing target proteins via IPTG or infection with Lambda CE6 phage, and growing bacteriophage stocks. Additionally, it includes troubleshooting tips, media preparation instructions, and an academic assurance letter regarding the use of T7 technology.
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Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the recommended storage temperature for these competent cells?
The competent cells must be stored at -80°C immediately upon arrival and should not be stored in liquid nitrogen.
How long should the heat pulse last during the transformation protocol?
Optimal results are achieved when reactions are heat-pulsed for 45-50 seconds.
What is the recommended storage temperature for these competent cells?
The competent cells must be stored at -80°C and should not be kept in liquid nitrogen.
What are the antibiotic resistances of these strains?
All three BL21-Gold-derived expression strains are resistant to tetracycline, and the BL21-Gold(DE3)pLysS strain is also resistant to chloramphenicol.