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Agilent Polaris 5u C18-A Antidiabetics Application Note
This document is an application note from Agilent Technologies regarding the analysis of antidiabetics. It references the acquisition of Varian, Inc. by Agilent and directs users to the company website for further information.
The note specifies the use of the Polaris 5u C18-A column (Part nr. A2000150X046) with dimensions of 150 x 4.6mm. The recommended eluent is a mixture of MeCN and 75 mM (NH4)3PO4 at pH 4.4 in a 70:30 ratio, with a flow rate of 21.0 ml/min and UV detection at 254nm. The chromatogram identifies glyburide as a peak.
Agilent Polaris 5u C18-A specifications
| Column | Polaris 5u C18-A, 150 x 4.6mm |
|---|---|
| Part Number | A2000150X046 |
| Eluent | MeCN : 75 mM (NH4)3PO4, pH 4.4, 70 : 30 |
| Flow Rate | 21.0 ml/min |
| Detection | UV @ 254nm |
Source: Agilent Polaris 5u C18-A Antidiabetics Application Note
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Questions answered in these manuals
Which column is used for the antidiabetics analysis?
The Polaris 5u C18-A column is used.
From: Agilent Polaris 5u C18-A Antidiabetics Application Note
What is the part number for the specified column?
The part number is A2000150X046.
From: Agilent Polaris 5u C18-A Antidiabetics Application Note
What column is used for the cysteine underivatized application?
The application uses a Polaris 5u C18-A column with dimensions 250 x 4.6mm.
From: Agilent Polaris 5u C18-A Cysteine Underivatized Application Note
What are the recommended eluent conditions?
Eluent A is 50 mM NH4H2PO4 + 1 mM sodium heptane sulfonate at pH 2.5, and Eluent B is MeCN.
From: Agilent Polaris 5u C18-A Cysteine Underivatized Application Note