Key facts
- Brand
- Agilent
- Model
- G6410A
- Documents
- 6
- Languages
- EN
- Last added
- 2012-07-02
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Agilent G6410A Triple Quadrupole Mass Spectrometer Amphetamine Analysis Application Note
This document presents a fast, sensitive, and validated technique for confirming the presence of amphetamine drugs in whole blood. The method utilizes an extractive alkylation technique followed by reverse-phase LC/MS/MS using the Agilent G6410A Triple Quadrupole Mass Spectrometer with electrospray ionization.
The analysis covers amphetamine, methamphetamine, MDA, and MDMA in a concentration range of approximately 15 to 1,000 ng/mL. The method demonstrates excellent linearity (R² > 0.999) with elution times for analytes and internal standards less than or equal to 3.6 minutes.
Agilent G6410A specifications
| Analyte Range | 15 to 1,000 ng/mL |
|---|---|
| Elution Time | <= 3.6 minutes |
| Column Temperature | 60°C |
| Flow Rate | 0.7 mL/min |
| Injection Volume | 2uL |
| Ionization Mode | Positive ESI |
Source: Agilent G6410A Triple Quadrupole Mass Spectrometer Amphetamine Analysis Application Note
All Agilent G6410A documents by type
How to use the product for one specific task, with worked examples.
- Agilent G6410A Triple Quadrupole Mass Spectrometer Amphetamine Analysis Application Note 8 p
- Agilent 1200 LC/G6410A Pharma Analysis Application Note 12 p
- Agilent Multiresidue Analysis of 301 Pesticides in Food Samples by LC/Triple Quadrupole Mass Spectrometry Manual 20 p
- Agilent G6410AA LC/MS/MS Aflatoxin Determination Application Note 8 p
- Agilent G6410AA LC/MS Application Note: Chloramphenicol Analysis in Food 8 p
- Agilent G6410AA Triple Quadrupole Mass Spectrometer Pesticide Determination Application Note 12 p
Questions answered in these manuals
Which amphetamine drugs are analyzed in this method?
The method analyzes amphetamine, methamphetamine, methylenedioxyamphetamine (MDA), and methylenedioxymethamphetamine (MDMA).
From: Agilent G6410A Triple Quadrupole Mass Spectrometer Amphetamine Analysis Application Note
What is the tolerance for ion ratio confirmation?
The overall ion ratio of the qualifier to the quantifier ions must fall within a +/- 20% tolerance.
From: Agilent G6410A Triple Quadrupole Mass Spectrometer Amphetamine Analysis Application Note
How many pharmaceuticals are analyzed in positive ion mode?
Nineteen pharmaceuticals are analyzed in positive ion mode.
From: Agilent 1200 LC/G6410A Pharma Analysis Application Note
What is the detection limit for the analytes?
Good linearity was observed for analytes from 1 pg to 1 ng on column.
From: Agilent 1200 LC/G6410A Pharma Analysis Application Note
How many pesticides were tested in this study?
The methodology was developed to screen and confirm the presence of 301 pesticides.
What is the limit of detection (LOD) achieved for most compounds?
90% of the compounds could be identified with a limit of detection of 0.01 mg/kg or below.