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Agilent ZORBAX Eclipse Plus C18 Application Note for Simvastatin Analysis
This document details the optimization of a USP method for analyzing simvastatin tablets. The original method utilizes an Agilent ZORBAX Eclipse Plus C18 5 µm column (250 mm x 4.6 mm) with a 10-minute analysis time.
The note demonstrates how to modify this method using smaller particle sizes and shorter columns, specifically the Rapid Resolution Eclipse Plus C18 (3.5 µm) and Rapid Resolution HT Eclipse Plus C18 (1.8 µm). These modifications significantly reduce analysis time and solvent usage while maintaining performance requirements. This manual is written in Japanese.
Agilent ZORBAX Eclipse Plus C18 specifications
| Original Column Part Number | 959990-902 |
|---|---|
| Rapid Resolution Column Dimensions | 4.6 mm x 100 mm, 3.5 µm |
| Flow Rate | 1.5 mL/min |
| Column Temperature | 45 °C |
| Detection Wavelength | 238 nm |
Source: Agilent ZORBAX Eclipse Plus C18 Application Note for Simvastatin Analysis
All Agilent ZORBAX Eclipse Plus C18 documents by type
How to use the product for one specific task, with worked examples.
- Agilent ZORBAX Eclipse Plus C18 Application Note for Simvastatin Analysis 12 p
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- Agilent ZORBAX Eclipse Plus C18 Improved Simvastatin Analysis Application Note 12 p
- Agilent ZORBAX Eclipse Plus C18 Application Note for Saponin Analysis 8 p
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- Agilent ZORBAX Eclipse Plus C18 Amino Acid Analysis Application Note 16 p
Ratings, dimensions and ordering codes, for selection and design work.
Questions answered in these manuals
What is the analysis time for the original USP method?
The original USP method requires a 10-minute analysis time.
From: Agilent ZORBAX Eclipse Plus C18 Application Note for Simvastatin Analysis
What is the required flow rate for the Simvastatin analysis method?
The method requires a flow rate of 1.5 mL/min.
From: Agilent ZORBAX Eclipse Plus C18 Application Note for Simvastatin Analysis
What is the maximum pressure limit for the Agilent Poroshell 120 EC-C18 column?
The maximum pressure limit for the Agilent Poroshell 120 EC-C18 column is 356 bar.
From: Agilent Poroshell 120 EC-C18 and ZORBAX Eclipse Plus C18 Method Transfer Technical Overview
What is the flow rate used in the generic gradient analysis described in the document?
The flow rate used in the generic gradient analysis is 2.0 mL/min.
From: Agilent Poroshell 120 EC-C18 and ZORBAX Eclipse Plus C18 Method Transfer Technical Overview
What is the maximum operating pressure for these columns?
The maximum operating pressure is 600 bar (9000 psi).
What pH range can these columns operate in?
These columns are designed for use over a pH range of 2-9.