Key facts
- Brand
- Agilent
- Model
- ZORBAX 300 SB-C8
- Documents
- 8
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Zorbax 300SB-C8 HPLC Column Datasheet
The Agilent Zorbax 300SB-C8 is a microparticulate column packing designed specifically for reversed-phase high-performance liquid chromatography (HPLC) of peptides and proteins. It features StableBond packing made by chemically bonding a sterically-protected octyl stationary phase to ultra-high-purity, porous-silica microspheres.
Read onlineAgilent ZORBAX 300 SB-C8 specifications
| Particle Pore Size | 300A |
|---|---|
| Maximum Operating Pressure (up to 9.4mm ID) | 400 bar (6000 psi) |
| Maximum Recommended Operating Temperature | 80°C |
| Recommended pH Range | pH 1.0 to 8.0 |
All Agilent ZORBAX 300 SB-C8 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
- Agilent ZORBAX 300SB-C8 Column Aging Application Note 2 p
- Agilent ZORBAX 300SB-C8 Effect of Particle Size on Column Efficiency Technical Note 2 p
- Agilent ZORBAX 300SB-C8 (3.5 m) Micro Flow Cell Comparison Application Note 2 p
- Agilent ZORBAX 300SB-C8 Preparative Separation Application Note 2 p
- Agilent ZORBAX 300 SB-C8 and SB-CN Stationary-Phase Selectivity Comparison 2 p
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the Zorbax 300SB-C8 primarily designed for?
It is designed specifically for reversed-phase HPLC of peptides and proteins.
Is the Zorbax 300SB-C8 suitable for low pH conditions?
Yes, it is particularly well suited for use with aggressive mobile phases at low pH (e.g., pH < 2).
Does silanol content change after exposure to low-pH eluent?
No, the silanol content does not change even after 18 liters of low-pH eluent.
What is the purpose of this test regarding peptides?
It serves as a very sensitive neutral-pH test of silanol accessibility.
How does reducing the particle size of a column packing affect separation efficiency?
Smaller particle size improves the efficiency of a separation without increasing run time, column length, or flow rate.
From: Agilent ZORBAX 300SB-C8 Effect of Particle Size on Column Efficiency Technical Note
What specific proteins were used to demonstrate the effect of particle size on separation?
The document lists Met Enkephalin, Leu ENkephalin, Angiotensin II, Neurotensin, RNase, Insulin, Lysozyme, Calmodulin, Myoglobin, and Carbonic Anhydrase.
From: Agilent ZORBAX 300SB-C8 Effect of Particle Size on Column Efficiency Technical Note