Key facts
- Brand
- Agilent
- Model
- Star 9080
- Documents
- 4
- Languages
- EN
- Last added
- 2012-06-12
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Agilent Star 9080 EC Detector Catecholamine Analysis Application Note
This document details the analysis of catecholamines (noradrenaline, adrenaline, and dopamine) using the Varian Star 9080 Amperometric Electrochemical Detector. It specifies the standard separation conditions, including the use of an ODS column, a mobile phase containing phosphoric acid, citric acid, EDTA, and octane sulphonic acid, and a flow rate of 1.0 mL/min.
Read onlineAgilent Star 9080 specifications
| Detector Type | Amperometric Electrochemical Detector |
|---|---|
| Flow Rate | 1.0 mL/min |
| Column Dimensions | 150 x 4.6 mm |
| Injection Volume | 20 uL |
| Flowcell Electrode | 2.74 mm Glassy Carbon |
| Reference Electrode | Ag/AgCl filled with saturated KCI/AgCl |
| E-cell Potential | 800 mV |
| I-cell Current | 3 nA |
Source: Agilent Star 9080 EC Detector Catecholamine Analysis Application Note
All Agilent Star 9080 documents by type
How to use the product for one specific task, with worked examples.
- Agilent Star 9080 EC Detector Catecholamine Analysis Application Note 4 p
- Varian Star 9080 Amperometric Electrochemical Detector Amino Acid Neurotransmitter Analysis Application Note 2 p
- Varian Star 9080 Pulsed Amperometric Detection of Disaccharides Application Note 1 p
- Varian Star 9080 EC Detector Analysis of Phenols in Water 2 p
Questions answered in these manuals
What is the typical S/N ratio for norepinephrine measured with a VT-03 glassy carbon flowcell?
A typical S/N ratio for norepinephrine measured with a VT-03 glassy carbon flowcell is 53000.
From: Agilent Star 9080 EC Detector Catecholamine Analysis Application Note
What is the concentration detection limit based on three times the noise?
The concentration detection limit based on three times the noise is 56 pmol/L.
From: Agilent Star 9080 EC Detector Catecholamine Analysis Application Note
What is the injection amount for each compound in this analysis?
The amount injected is 20 pmol (1 pmol/L) for each compound.
What are the identified peaks in the chromatogram?
Peaks correspond to serine, glycine, taurine, glutamate, arginine, alanine, OPA reagent, and GABA.
What disaccharides can be detected using the Varian Star 9080?
The detector is used for the pulsed amperometric detection of alpha-lactose, sucrose, and maltose.
From: Varian Star 9080 Pulsed Amperometric Detection of Disaccharides Application Note
What is the required flowrate for the Dionex CarboPac PA1 column?
The procedure specifies a flowrate of 1.0 mL/min.
From: Varian Star 9080 Pulsed Amperometric Detection of Disaccharides Application Note