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Agilent ProSEC 300S Columns for Protein SEC Data Sheet
The document details the ProSEC 300S columns, which contain hydrophobic silica particles designed for the separation of biomolecules including proteins and peptides. It covers installation requirements, such as the use of specific stainless steel tubing and compatible connectors to minimize dead volume. The text also specifies that column connections must be made using Parker compatible 1/16 in nuts and ferrules.
Read onlineAgilent ProSEC 300S specifications
| Typical operating pressure | 900 psi (60 bar) |
|---|---|
| Maximum operating pressure | 3700 psi (250 bar) |
| Maximum operating temperature | 40 °C |
| Efficiency | > 75,000 plates |
Source: Agilent ProSEC 300S Columns for Protein SEC Data Sheet
All Agilent ProSEC 300S documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
- Agilent Analysis of Albumin Proteins using ProSEC 300S Columns Manual 2 p
- Agilent ProSEC 300S Columns Calibration Application Note 2 p
- Agilent Effect of pH on Protein Size Exclusion Chromatography Application Note 2 p
- Agilent ProSEC 300S Columns Calibration Application Note 2 p
- Agilent Effect of pH on Protein Size Exclusion Chromatography Application Note 2 p
Questions answered in these manuals
What eluent pH range is recommended for ProSEC 3008 columns?
Buffered eluent systems within the pH range 5.0-7.5 are recommended.
From: Agilent ProSEC 300S Columns for Protein SEC Data Sheet
Can organic solvents be used with ProSEC 3008 columns?
Yes, organic solvents and organic/aqueous mixtures may be used.
From: Agilent ProSEC 300S Columns for Protein SEC Data Sheet
What is the physical size of the ProSEC 3008 column used in this analysis?
The column dimensions are 300 x 7.5 mm.
From: Agilent Analysis of Albumin Proteins using ProSEC 300S Columns Manual
What flow rate was used during the protein analysis?
The sample was analyzed at a flow rate of 1.0 mL/min.
From: Agilent Analysis of Albumin Proteins using ProSEC 300S Columns Manual
What proteins were used for calibration?
The calibration used Thyroglobulin, y-Globulin, Bovine serum albumin, Ovalbumin, Carbonic anhydrase, Myoglobulin, Cytochrome C, and Bacitracin.
From: Agilent ProSEC 300S Columns Calibration Application Note
What does the calibration curve show?
The calibration curve shows a linear relationship between the log of the molecular weight and the retention time.
From: Agilent ProSEC 300S Columns Calibration Application Note