Agilent DB-EUPAH and DB-5ms High Efficiency GC Column Selection Guide

About this manual

This application note compares the performance of Agilent J&W DB-EUPAH and DB-5ms High Efficiency GC columns for the analysis of 24 regulated Polycyclic Aromatic Hydrocarbons (PAHs). The DB-EUPAH column is recommended when the resolution of benzo[b,j,k]fluoranthene isomers is required, achieving separation of all 24 analytes in under 28 minutes. The DB-5ms column is an excellent choice when reporting the sum of benzofluoranthene isomers is sufficient, offering a faster analysis time of under 22 minutes for 23 of the 24 analytes.

What's inside

• Introduction
• Regulated PAH Compounds
• Chromatographic Conditions DB-EUPAH
• Chromatographic Conditions DB-5ms
• Flow Path Supplies
• Discussion of Results
• Conclusions

Specifications

Frequently asked questions

Which column is recommended for separating benzo[b,j,k]fluoranthene isomers?

The Agilent J&W DB-EUPAH column is recommended when separation of benzo[b,j,k]fluoranthene isomers is required.

How long does the analysis take when using the DB-EUPAH column?

The separation of all 24 regulated PAHs is achieved in under 28 minutes.

What is the recommended column for separating benzo[b,j,k]fluoranthene isomers?

The Agilent J&W DB-EUPAH column is recommended when separation of these isomers is required.

What injection volume should be used for the high efficiency GC format?

The injection volume was reduced to 0.5 pL in order to scale the separation.