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Agilent Trace-Level Analysis of Melamine in Milk Products on Agilent 7890A/5975C GC/MSD Using a New Agilent J W DB-5ms Ultra Inert Column SampliQ SCX Cartridges Manual
The document presents a Gas Chromatography/Mass Spectrometry (GC/MS) method for the quantitative determination and confirmation of melamine residues in milk products. The sample preparation involves cleaning up milk samples using Agilent's new SampliQ SCX SPE cartridges followed by derivatization.
The derived extracts are analyzed by GC/MS on a new Agilent J&W DB-5ms Ultra Inert column operating in synchronous SIM/scan mode. The method demonstrates good linearity, high recoveries over 80 percent, and a low limit of detection (LOD) of 0.0025 mg/kg suitable for trace-level analysis.
Agilent 7890A-5975C specifications
| Column Dimensions | 30 m x 0.25 mm x 0.25 µm |
|---|---|
| Inlet Temperature | 250 °C |
| Oven Program Start | 75°C for 1 min |
| Oven Ramp Rate | 30 °C/min to 300 °C |
| Post-run Hold Time | 5 minutes at 280 °C |
| MS Source Temperature | 230 °C |
| Linearity Range | 10 to 200 µg/g |
| Correlation Coefficient | > 0.9996 |
All Agilent 7890A-5975C documents by type
How to use the product for one specific task, with worked examples.
- Agilent Trace-Level Analysis of Melamine in Milk Products on Agilent 7890A/5975C GC/MSD Using a New Agilent J W DB-5ms Ultra Inert Column SampliQ SCX Cartridges Manual 6 p
- Agilent Rapid Screening of Melamine Cyanuric Acid in Milk Products Using Agilent J W HP-5ms GC Column Agilent 7890A/5975C GC/MSD with Column Backflushing Manual 8 p
- Agilent 7890A-5975C and 1200SL-6410 Melamine Cyanuric Acid Analysis Application Note 8 p
Questions answered in these manuals
What is the lowest concentration of melamine this method can detect in milk?
The limit of detection for melamine in the milk matrix is 0.0025 mg/kg.
What is the linear concentration range for this melamine analysis method?
The method shows good linearity in the range of 0.025 to 2.000 pg/mL.
How much time does the backflushing method save compared to conventional baking?
The method reduces the GC run time from more than 70 minutes to 14.5 minutes.
What is the recovery rate for melamine and cyanuric acid in spiked samples?
The recoveries are greater than 95%, with specific results of 96.1% for cyanuric acid and 95.6% for melamine.
What is the detection limit for the GC/MS screening method?
The detection limit for the GC/MS screening method is 10 ug/g.
From: Agilent 7890A-5975C and 1200SL-6410 Melamine Cyanuric Acid Analysis Application Note
Does the LC/MS/MS method require derivatization?
No, the LC/MS/MS method requires only extraction and then a simple isocratic elution without derivatization.
From: Agilent 7890A-5975C and 1200SL-6410 Melamine Cyanuric Acid Analysis Application Note