Key facts
- Brand
- Agilent
- Model
- 1100 HPLC
- Documents
- 2
- Languages
- EN
- Last added
- 2012-07-02
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Agilent Technologies Use of Temperature to Increase Resolution in the Ultrafast HPLC Separation of Proteins with ZORBAX Poroshell 300SB-C8 HPLC Columns. Optimization of the Agilent 1100 HPLC System f
This document provides a technical overview of optimizing the Agilent 1100 HPLC system for the separation of proteins and peptides using ZORBAX Poroshell superficially porous particle technology. It highlights how this technology allows for faster separations with reduced peak broadening compared to totally porous particles, even at higher flow rates. The text explains the mechanism of the Poroshell particle structure, which consists of a porous silica crust around a nonporous core, enabling molecules to diffuse in and out quickly.
Read onlineAgilent 1100 HPLC specifications
| Column (Analytical) | 150 x 3.0 mm ZORBAX SB-C18, 5 µm |
|---|---|
| Column (Semipreparative) | 50 x 9.4 mm ZORBAX SB-C18, 5 µm |
| Flow rate (Semipreparative) | 7.0 mL/min |
Source: Agilent 1100 LC/MSD Application Note: Identification and Isolation of DSP-Toxins
All Agilent 1100 HPLC documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
How should flow rate be adjusted when switching from a 2.1-mm to a 1.0-mm i.d. column?
The flow rate should be reduced 5-fold to maintain the same relative flow rate (linear velocity).
What is the maximum operating temperature for ZORBAX Poroshell 300SB-C18 columns?
The columns are stable at temperatures up to 80 °C.
What toxins are identified in this application note?
The document focuses on Okadaic Acid (OA) and Dinophysistoxin-1 (DTX-1).
From: Agilent 1100 LC/MSD Application Note: Identification and Isolation of DSP-Toxins
How much does the detection limit improve with LC/MS?
Detection limits are decreased by a factor of 3-5 compared to LC with fluorescence detection.
From: Agilent 1100 LC/MSD Application Note: Identification and Isolation of DSP-Toxins