Key facts
- Brand
- Agilent
- Model
- ZORBAX SB-C18
- Category
- Other Electronics
- Documents
- 13
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Ultra-fast liquid chromatography using the Agilent 1100 Series HPLC system 1.8-um ZORBAX SB C18 Rapid Resolution HT columns Manual
This document describes the recommended system configuration to achieve very fast analysis and cycle times using ZORBAX 1.8-um Rapid Resolution High Throughput (RRHT) columns on the Agilent 1100 Series liquid chromatograph. It details how to reduce analysis time to seconds without compromising resolution or chromatographic performance.
Read onlineAgilent ZORBAX SB-C18 specifications
| Analysis time | 29 seconds |
|---|---|
| Peak width | 0.5 seconds |
| Backpressure | 300 bar |
| Column particle size | 1.8 um |
| Delay volume | 300 pL |
| Injection speed | < 30 seconds |
| Flow cell volume | 500-nL |
All Agilent ZORBAX SB-C18 documents by type
- Agilent Ultra-fast liquid chromatography using the Agilent 1100 Series HPLC system 1.8-um ZORBAX SB C18 Rapid Resolution HT columns Manual 12 p
- Agilent ZORBAX SB-C18 vs Eclipse XDB-C18 Application Note 2 p
- Agilent ZORBAX SB-C18 Column Stability Application 2 p
- Agilent ZORBAX SB-C18 Rapid-Resolution Column Application Note 2 p
- Agilent ZORBAX SB-C18 Reversed-Phase Separation Application Note 2 p
- Agilent ZORBAX SB-C18 Water-Soluble Vitamins USP 23 Application Leaflet 2 p
- Agilent ZORBAX SB-C18 High-Efficiency Separation of Natural Anthocyanins Application Note 2 p
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the minimum configuration for ultra-fast analysis?
The minimum configuration consists of the Agilent 1100 Series binary pump, well-plate autosampler, thermostatted column compartment, and ZORBAX 1.8-um RRHT columns.
What is the benefit of overlapped injections (OD)?
Overlapped injection allows the preparation of the next injection while the current injection is being completed, increasing sample throughput.
What is the maximum operating pressure for these columns?
The maximum operating pressure is 600 bar (9000 psi).
What is the recommended storage solvent for long term storage?
Long term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
What technology is used in the ZORBAX SB-C18 column?
It uses StableBond C18 packing made by chemically bonding a sterically-protected C18 stationary phase to high purity silica spheres.
What is the particle size of this column?
The column features sub-2-micron particles designed to reduce or eliminate strong adsorption.
