Key facts
- Brand
- Agilent
- Model
- Bio SEC-3
- Documents
- 4
- Languages
- ES · EN
- Last added
- 2012-07-09
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Agilent Technologies BioHPLC Column Portfolio Brochure
This document introduces the Agilent BioHPLC portfolio, specifically focusing on Size Exclusion (SEC) and Ion Exchange (IEX) chromatography columns. It highlights two main product lines: the Bio SEC-3 series with 3 µm particles for high-resolution separations of biomolecules like proteins and polymers, and the Bio SEC-5 series with 5 µm particles designed for maximum efficiency and stability. This manual is written in Spanish.
Read onlineAgilent Bio SEC-3 specifications
| Particle Size (Bio SEC-3) | 3 µm |
|---|---|
| Particle Size (Bio SEC-5) | 5 µm |
| Recommended Operating Temperature Range | 10 -30 °C |
Source: Agilent Technologies BioHPLC Column Portfolio Brochure
All Agilent Bio SEC-3 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What are the recommended applications for Agilent BioHPLC columns?
Recommended applications include antibody aggregation analysis, protein separation in cell lysates, and mixtures of proteins, natural polymers, nanomaterials, oligonucleotides, and polysaccharides.
From: Agilent Technologies BioHPLC Column Portfolio Brochure
What is the maximum operating temperature for Agilent Bio SEC-3 and Bio SEC-5 columns?
The recommended interval is 10 to 30 degrees Celsius, with a maximum limit of 80 degrees Celsius.
From: Agilent Technologies BioHPLC Column Portfolio Brochure
What biomolecules are suitable for separation with Agilent Bio SEC-3 columns?
These columns are designed for antibodies, proteins, peptides, oligonucleotides, polysaccharides and other large bio-molecules.
What is the recommended pH range for using these columns?
The columns can be used in the range pH 2–8.5 to optimize performance and lifetime.
What system was used for the rEPO separation?
The Agilent 1260 Infinity Bio-inert Quaternary LC system.
From: Agilent Bio SEC-3 Application Note for rEPO Separation
What is the recommended flow rate for intact rEPO separation?
The optimized flow rate was 0.35 mL/min.
From: Agilent Bio SEC-3 Application Note for rEPO Separation