Key facts
- Brand
- Agilent
- Model
- 1200L LC
- Documents
- 6
- Languages
- EN
- Last added
- 2012-06-12
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Agilent 1200L LC/MS/MS Application Note for Dinitro Flavonoid Quantitation
This document details a pharmacokinetic evaluation method for determining the profile of a novel dinitro-substituted flavone (DNF) in rat plasma. The study utilized oral administration and analyzed samples to observe concentration changes over time, noting phenomena such as entero-hepatic recirculation.
The analysis was performed using an Agilent 1200L LC/MS/MS system equipped with an ESI source. The method involves extracting DNF from plasma using hexane:chloroform and analyzing it via Selected Reaction Monitoring (SRM). Specific HPLC conditions, MS parameters, and scan settings for the analyte and internal standard are provided.
Agilent 1200L LC specifications
| Detection Limit | 15 picograms |
|---|---|
| Therapeutic Level | 0.5 - 2.0 ng/mL |
| Column Dimensions | 50 mm x 1.0 mm |
| Drying Gas Temperature | 200 °C |
| Detection Mode | Negative Ion ESI |
Source: Agilent 1200L LC/MS Application Note 7 Digoxin Quantitation
All Agilent 1200L LC documents by type
How to use the product for one specific task, with worked examples.
- Agilent 1200L LC/MS/MS Application Note for Dinitro Flavonoid Quantitation 2 p
- Agilent 1200L LC/MS ESI and APCI Ionization Application Note 2 p
- Agilent 1200L LC/MS/MS Application Note: Femtogram Sensitivity of Raclopride and Ritanserin 2 p
- Agilent 1200L LC/MS Application Note 7 Digoxin Quantitation 2 p
- Agilent 1200L LC/MS/MS Application Note for Cyclosporin A Analysis 2 p
- Varian 1200L LC/MS/MS Dynamic Range Application Note 2 p
Questions answered in these manuals
What system was used for the quantitation of DNF in this study?
A Varian (now Agilent) 1200L LC/MS/MS system equipped with an ESI source.
From: Agilent 1200L LC/MS/MS Application Note for Dinitro Flavonoid Quantitation
What internal standard was used in the analysis?
Flavone was used as the internal standard.
From: Agilent 1200L LC/MS/MS Application Note for Dinitro Flavonoid Quantitation
What flow rate is used for the ESI source?
The flow rate for ESI is 10 pL/min.
From: Agilent 1200L LC/MS ESI and APCI Ionization Application Note
What flow rate is used for the APCI source?
The flow rate for APCI is 20 pL/min.
From: Agilent 1200L LC/MS ESI and APCI Ionization Application Note
What signal-to-noise ratio was observed for Raclopride at its lowest detectable level?
At the lowest concentration of 100 pg/mL, the S/N ratio is approximately 10:1.
From: Agilent 1200L LC/MS/MS Application Note: Femtogram Sensitivity of Raclopride and Ritanserin
What is the detection limit for Digoxin in this method?
The method achieves quantitative measurement down to 15 picograms of Digoxin.
From: Agilent 1200L LC/MS Application Note 7 Digoxin Quantitation