Key facts
- Brand
- Agilent
- Model
- 500-MS Ion Trap LC
- Documents
- 3
- Languages
- EN
- Last added
- 2012-06-12
Start here
Agilent Application Note 01636: Mass Spectrometry With Evaporative Light Scattering Detection for Pharmaceuticals
This document describes an analytical method combining Mass Spectrometry (MS) with Evaporative Light Scattering Detection (ELSD). It addresses limitations in standard MS analysis, such as poor ionization of certain analytes and matrix effects. The note details how coupling these detectors allows for simultaneous detection or serial/selected detection using a divert valve.
Three specific pharmaceutical applications are presented: the analysis of Haloperidol and its metabolites, the differentiation of Diazepam and Verapamil responses, and the quantitation of Ibuprofen in the presence of B-cyclodextrin. The text provides specific instrument configurations, HPLC conditions, and parameter settings for both the MS and ELSD systems.
All Agilent 500-MS Ion Trap LC documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
How does ELSD compare to MS in terms of response for Diazepam and Verapamil?
While these compounds show significantly different responses in full scan MS, they exhibit a relatively equal response in the ELSD detector.
What is the flow rate range compatible with ESI or APCI for this system?
The ELSD has similar operating parameters to LC/MS, utilizing flow rates in the range of 0.2-0.5 mL/min.
What is the purpose of this application note?
This document describes a method to separate and analyze the components of the Ethomeen T/20H emulsifier using LC/MS.
From: Agilent 500-MS Ion Trap LC/MS Application Note for Ethomeen T/20H Characterization
What instrument is used for the analysis?
The analysis uses a Varian 500-MS Ion Trap LC/MS equipped with an ESI source.
From: Agilent 500-MS Ion Trap LC/MS Application Note for Ethomeen T/20H Characterization
What is the total run time for the analysis method?
The method is completed in less than five minutes.
From: Agilent 500-MS Ion Trap LC/MS Analysis of Isocyanate Derivatives Application Note
What is the lowest limit of detection reported for any compound?
The lowest limit of detection is 75 ppt for HDI.
From: Agilent 500-MS Ion Trap LC/MS Analysis of Isocyanate Derivatives Application Note