Key facts
- Brand
- Agilent
- Model
- Poroshell 120 EC-C18
- Documents
- 10
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Poroshell 120 EC-C18 and ZORBAX Eclipse Plus C18 Method Transfer Technical Overview
This document provides a technical overview of transferring chromatographic methods from larger totally porous particles to superficially porous particles, specifically comparing Agilent Poroshell 120 EC-C18 and Agilent ZORBAX Eclipse Plus C18 columns. It highlights the benefits of using superficially porous particles for time savings and reduced backpressure while maintaining high efficiency.
Read onlineAgilent Poroshell 120 EC-C18 specifications
| Column Type | Agilent Poroshell 120 EC-C18 |
|---|---|
| Analysis Time | less than 3 minutes |
| Pressure Limit | below 400 bar |
| Flow Rate Range | 0.5 mL/min to 3.5 mL/min |
| Column Length Options | 4.6 x 100 mm and 4.6 x 50 mm |
Source: Agilent Poroshell 120 EC-C18 Columns Application Note
All Agilent Poroshell 120 EC-C18 documents by type
How to use the product for one specific task, with worked examples.
- Agilent Poroshell 120 EC-C18 Columns Application Note 8 p
- Agilent Fast Analysis of Zanthoxylum nitidum Using Agilent Poroshell 120 EC-18 Columns 4 p
- Agilent SAMHSA-Compliant LC/MS/MS Analysis of 6-Acetylmorphine in Urine with Agilent Bond Elut Plexa PCX Agilent Poroshell 120 Manual 6 p
- Agilent SAMHSA-Compliant LC/MS/MS Analysis of Benzoylecgonine in Urine with Agilent Bond Elut Plexa PCX Agilent Poroshell 120 Manual 6 p
- Agilent Bond Elut Plexa PCX and Poroshell 120 Opiate Analysis Application Note 8 p
- Agilent SAMHSA-Compliant LC/MS/MS Analysis of Phencyclidine in Urine with Agilent Bond Elut Plexa PCX Agilent Poroshell 120 Manual 6 p
- Agilent SAMHSA-Compliant LC/MS/MS Analysis of 11-nor-9-carboxy-D9-Tetrahydrocannabinol in Urine with Agilent Bond Elut Plexa PCX Agilent Poroshell 120 Manual 6 p
Questions answered in these manuals
What is the maximum pressure limit for the Agilent Poroshell 120 EC-C18 column?
The maximum pressure limit for the Agilent Poroshell 120 EC-C18 column is 356 bar.
From: Agilent Poroshell 120 EC-C18 and ZORBAX Eclipse Plus C18 Method Transfer Technical Overview
What is the flow rate used in the generic gradient analysis described in the document?
The flow rate used in the generic gradient analysis is 2.0 mL/min.
From: Agilent Poroshell 120 EC-C18 and ZORBAX Eclipse Plus C18 Method Transfer Technical Overview
What is the analysis time for the new method?
The new separation reduces the analysis time from 20 minutes to less than 3 minutes.
Does the method require changes to sample preparation?
No, the method should not require any changes to sample preparation since both columns use 2 um frits.
What is the analysis time for the optimized method?
The analysis time fell to approximately one-tenth of the original analysis time, reaching below 4 minutes.
From: Agilent Fast Analysis of Zanthoxylum nitidum Using Agilent Poroshell 120 EC-18 Columns
Can this method be run on a traditional HPLC instrument?
Yes, the backpressure is below 400 bar, allowing it to be run on a traditional HPLC instrument.
From: Agilent Fast Analysis of Zanthoxylum nitidum Using Agilent Poroshell 120 EC-18 Columns