Key facts
- Brand
- Agilent
- Model
- 1200 Series Rapid Resolution LC
- Category
- Other Electronics
- Documents
- 18
- Languages
- DE · EN
- Last added
- 2012-07-06
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Agilent 1200 Series Rapid Resolution LC and Rapid Resolution LC/MS Optimization Guide
This guide explains how to optimize Agilent 1200 Series Rapid Resolution LC and LC/MS systems using sub-2-micron particles for faster chromatography and higher resolution. It covers system design, column regeneration, resolution enhancement, sensitivity improvement, and column blockage prevention. The guide also includes optimization for Agilent 6000 series mass spectrometers and application examples for specific compound analyses.
The guide is structured into sections on theory, system optimization, mass spectrometer instrumentation, application examples, and appendices, with detailed explanations of van Deemter equations, particle size effects, and practical implementation steps.
Agilent 1200 Series Rapid Resolution LC specifications
| Sample throughput | 2000 samples per day |
|---|---|
| Peak capacity | more than 600 peaks |
| Column length | 150 mm |
| Resolution improvement | 60% higher than HPLC |
| Speed improvement | up to 20 times faster than HPLC |
| Column inner diameter | 1 to 4.6 mm |
| Particle size | 1.5 to 10 um |
| Maximum temperature | 100°C |
| Maximum pressure | 600 Bar |
| Injection volume range | 0.1 to 100 ul |
Source: Agilent 1200 Series Rapid Resolution LC System Product Leaflet
All Agilent 1200 Series Rapid Resolution LC documents by type
- Agilent 1200 Series Rapid Resolution LC Technical Note 4 p
- Agilent 1200 Series Rapid Resolution LC System Technical Note 8 p
- Agilent 1200 Series RRLC Method Transfer Technical Note 8 p
- Agilent 1100 Series LC Upgrade to 1200 Series Technical Note 8 p
- Step-by-step upgrade of Agilent 1100 to 1200 Series Rapid Resolution LC systems Technical Note 8 p
- Agilent 1200 Series RRLC Supported Modules and Tasks Technical Note 8 p
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
How to use the product for one specific task, with worked examples.
- Agilent Fast Ultra-Fast Analysis Using the Agilent 1200 Series Rapid Resolution LC System Compared to a Conventional Agilent 1100 Series LC System 8 p
- Agilent Determination of Fluvastatin in Plasma Using the Agilent 6410B Triple Quadrupole LC/MS System and Agilent 1200 S 4 p
- Agilent 1200 Series RRLC and 6460 MS Application Note for Plasma Stability 8 p
- Agilent 1200 Series LC and 6210 TOF MS Application Note 8 p
- Agilent 1200 Series Rapid Resolution LC Herbicide Analysis Application Note 6 p
- Agilent 5990-3528EN- Running fast LC within USP limits Case study with USP pravastatin sodium chromatographic purity method using the Agilent 1200 Series Rapid Resolution LC system Application Note 8 p
- Agilent Determination of degradation products of Metoprolol tablets with the Agilent 1120 Compact LC after method development with the Agilent 1200 Series Rapid Resolution LC system Application Note 8 p
Questions answered in these manuals
How much faster is the Rapid Resolution LC System compared to HPLC?
The system is up to 20 times faster than conventional HPLC.
From: Agilent 1200 Series Rapid Resolution LC System Product Leaflet
Is the Rapid Resolution LC System compatible with HPLC methods?
Yes, it is fully compatible with HPLC methods.
From: Agilent 1200 Series Rapid Resolution LC System Product Leaflet
What is the maximum column temperature supported by the Agilent 1200 Series Rapid Resolution LC system?
The system's column compartment SL supports temperatures up to 100 °C.
From: Agilent 1200 Series Rapid Resolution LC Technical Note
How does increasing the column temperature affect the analysis time for a specific chromatographic run?
Increasing the column temperature from 40 °C to 95 °C shortened the analysis time to 0.4 min.
From: Agilent 1200 Series Rapid Resolution LC Technical Note
What is the maximum pressure the Agilent 1200 Series binary pump SL can handle?
The pump can be used up to pressures of 600 bars.
From: Agilent 1200 Series Rapid Resolution LC System Technical Note
What is the delay volume range in the standard configuration of the Agilent 1200 Series binary pump SL?
In the standard configuration, the delay volume is between 600 and 800 pL depending on the pressure.
From: Agilent 1200 Series Rapid Resolution LC System Technical Note