Key facts
- Brand
- Agilent
- Model
- 1200 Series RRLC
- Documents
- 10
- Languages
- EN
- Last added
- 2012-07-02
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Agilent 1200/6410 LC/MS Application Note for DMPK Quantification
This document details the development of a multi-MRM method for the rapid and selective quantification of drug compounds in cassette-dosing DMPK studies. It addresses the need for high-throughput screening by utilizing rapid resolution liquid chromatography (RRLC) to separate compounds and a triple quadrupole mass spectrometer operating in MRM mode for sensitive detection.
Read onlineAgilent 1200 Series RRLC specifications
| Column | Agilent ZORBAX SB C-18, 2.1 x 50 mm, 1.8 pm particle size |
|---|---|
| Flow rate | 1 mL/min |
| Injection volume | 1 pL |
| Column temperature | 50 °C |
| Source Temperature | 300 °C |
| Gas flow | 12 L/min |
| Nebulizer pressure | 60 psi |
| Capillary voltage | 4000 V |
| Dwell time | 35 ms |
| LOD for compound B | 100 fg (on-column) |
Source: Agilent 1200/6410 LC/MS Application Note for DMPK Quantification
All Agilent 1200 Series RRLC documents by type
How to use the product for one specific task, with worked examples.
- Agilent 1200/6410 LC/MS Application Note for DMPK Quantification 8 p
- Agilent 1200 Series RRLC 6410 LC Triple Quad Benzodiazepine Analysis Application Note 10 p
- Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4 4 p
- Agilent Improving Productivity in Early In Vitro ADME Part 3 Caco-2 Permeability Assay Application Note 4 p
- Agilent 1200 Series RRLC and 6410B LC/MS Application Note for Impurity D Quantification 8 p
- Agilent 1200 Series RRLC and 6460 MS Application Note for Plasma Stability 8 p
- Agilent 1200 Series RRLC Analysis of Phenolic Antioxidant and Erucamide Slip Additives in Polymer 8 p
- Agilent 1200 Series RRLC Application Note for Zuo Jin Pill Quantification 8 p
- Agilent 1200 Series RRLC AS Purification of PNA Application Note 4 p
- Agilent 1200 Series RRLC AS Peptide Purification Application Note 4 p
Questions answered in these manuals
How is ion suppression overcome in this method?
Ion suppression is overcome by using rapid resolution liquid chromatography (RRLC) to elute discrete compounds into the ion source.
From: Agilent 1200/6410 LC/MS Application Note for DMPK Quantification
What is the limit of detection for compound B?
A limit of detection of 100 fg on-column was determined for compound B with a signal-to-noise of 4.4.
From: Agilent 1200/6410 LC/MS Application Note for DMPK Quantification
What is the limit of quantitation for this benzodiazepine hair analysis method?
The limit of quantitation is 50 pg/mg of hair.
From: Agilent 1200 Series RRLC 6410 LC Triple Quad Benzodiazepine Analysis Application Note
Which Agilent instruments are used for this analysis?
The analysis uses an Agilent 1200 Series RRLC coupled with a 6410 LC Triple Quadrupole Mass Spectrometer.
From: Agilent 1200 Series RRLC 6410 LC Triple Quad Benzodiazepine Analysis Application Note
What equipment is used in the PPB assay described?
The study uses Agilent Equipment: 1200 Series RRLC, 6410 triple quadrupole LC/MS, CTC HTC-PAL autosampler, ZORBAX RRHT column, and MassHunter workstation software.
From: Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4
What was the incubation temperature for the plasma protein binding assay?
The incubation temperature was set to 37°C.
From: Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4