A quantitative analytical procedure for the determination of benzodiazepines and metabolites in hair has been developed and validated. The hair samples were washed, incubated, and any drugs present were quantified using mixed mode solid-phase extraction and liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) in positive electrospray ionization mode.
This document details a quantitative analytical procedure for determining benzodiazepines and their metabolites in hair. The method utilizes mixed-mode solid-phase extraction followed by liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) in positive electrospray ionization mode.
The procedure covers the analysis of 14 benzodiazepines and 5 metabolites, with a limit of quantitation of 50 pg/mg. The method was validated for linearity, precision, and accuracy over a concentration range of 50 to 1,000 pg/mg. Key features include the simultaneous monitoring of qualifying ions to ensure result confirmation, a critical requirement for forensic analysis.
| Limit of Quantitation | 50 pg/mg of hair |
|---|---|
| Analysis Range | 50 to 1,000 pg/mg |
| Instrument | Agilent 1200 Series RRLC; 6410 LC Triple Quadrupole Mass Spectrometer |
| Column | ZORBAX RRHT Eclipse XDB C18, 4.6 mm x 50 mm x 1.8 um |
| Ionization Mode | Electrospray ESI positive mode |
The limit of quantitation is 50 pg/mg of hair.
The analysis uses an Agilent 1200 Series RRLC coupled with a 6410 LC Triple Quadrupole Mass Spectrometer.
The procedure detects 14 benzodiazepines and 5 metabolites in hair.