This Application Note demonstrates the configuration of the Agilent 1200 Series Rapid Resolution LC (RRLC) analytical scale (AS) purification system and its optimized method for purification of peptides from solid-phase synthesis.
This document details the use of the Agilent 1200 Series Rapid Resolution LC (RRLC) analytical scale (AS) purification system to purify peptides synthesized via solid-phase synthesis. The authors demonstrate how to configure the system and apply an optimized method to separate peptides from truncated sequences generated during synthesis.
| Sample temperature | 4 °C |
|---|---|
| Column temperature | 45 °C |
| Flow rate (10 min method) | 1 mL/min |
| Flow rate (4 min method) | 2 mL/min |
| Purification time (optimized) | 4 minutes |
The Agilent 1200 Series Rapid Resolution LC (RRLC) analytical scale (AS) purification system.
The optimized method allows for purification within 4 minutes per peptide.