Key facts
- Brand
- Agilent
- Model
- StableBond SB-C18
- Documents
- 2
- Languages
- JA · EN
- Last added
- 2012-07-06
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Agilent ZORBAX StableBond SB-C18 LC Column Application Note for Oxycodone Analysis
This document details a method for analyzing oxycodone and its oxidation metabolites (noroxycodone, oxymorphone, noroxymorphone) in human plasma. The method combines high-speed liquid chromatography with mass spectrometry detection using an Agilent ZORBAX Rapid Resolution High Throughput StableBond SB-C18 column.
The procedure involves solid-phase extraction of spiked human plasma samples followed by analysis via LC/MS in electrospray positive ion mode. The method achieves good linearity (R² > 0.9900) and reproducibility, with a limit of detection for oxycodone at 0.5 ng/mL. This manual is written in Japanese.
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How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the minimum detection limit for oxycodone using this method?
The detection and quantification limit for oxycodone is 0.5 ng/mL.
From: Agilent ZORBAX StableBond SB-C18 LC Column Application Note for Oxycodone Analysis
What are the dimensions of the ZORBAX StableBond SB-C18 column used?
The column measures 2.1 mm in inner diameter and 50 mm in length.
From: Agilent ZORBAX StableBond SB-C18 LC Column Application Note for Oxycodone Analysis
What is the stated length of the StableBond SB-C18 column used in this method?
The column has a stated length of 150 mm.
From: Agilent StableBond SB-C18 Carbonyl Analysis Application Note
How long does the analysis of an air sample take using this method?
An air sample was analyzed in under 10 minutes.
From: Agilent StableBond SB-C18 Carbonyl Analysis Application Note