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Agilent 1290 Infinity LC 6530 Q-TOF Metabolite Identification Application Note
This document details a workflow for analyzing the metabolism of pharmaceutical drug candidates. It utilizes the Agilent 1290 Infinity LC system for rapid separation and the Agilent 6530 Accurate-Mass Q-TOF LC/MS system for fast acquisition of mass spectra. The identified metabolites are then analyzed using Agilent MassHunter Metabolite Identification software to generate reports.
Read onlineAgilent 6530 Accurate-Mass Q-TOF specifications
| Flow rate | 0.8 mL/min |
|---|---|
| Column temperature | 60 °C |
| Mass range | 100-1000 m/z |
| Acquisition rate | 5 spectra/s |
Source: Agilent 1290 Infinity LC 6530 Q-TOF Metabolite Identification Application Note
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Questions answered in these manuals
What software is used for metabolite identification?
Agilent MassHunter Metabolite Identification software is used for fast, software-assisted identification of main metabolites.
From: Agilent 1290 Infinity LC 6530 Q-TOF Metabolite Identification Application Note
What is the purpose of this application note?
This document describes a method for the rapid separation and identification of main metabolites generated from pharmaceutical drugs in in-vitro experiments.
From: Agilent 1290 Infinity LC 6530 Q-TOF Metabolite Identification Application Note
What PGRs are detected in positive mode?
The PGRs detected in positive mode are Chlormequat, d4 chlormequat, Daminozide, Zeatin, Kinetin, 6-Benzyl adenine, Indol Butyric acid, Forchlorfenuron, and Paclobutrazol.
What PGRs are detected in negative mode?
The PGRs detected in negative mode are Gibbrelic acid, Abscisic acid, and 2, 4 Dichlorphenoxy acetic acid.
What is the limit of quantitation (LOQ) for cystine in WBCs?
The LOQ of cystine in WBCs is 0.02 uM, or 40 fmol on-column.
From: Agilent 6530 Accurate-Mass Q-TOF LC/MS System Application Note
What is the dynamic range of the cystine calibration curve?
The cystine calibration curve shows excellent linearity in the dynamic range of 0.02 - 4 uM.
From: Agilent 6530 Accurate-Mass Q-TOF LC/MS System Application Note