Key facts
- Brand
- Agilent
- Model
- 6520 Q-TOF
- Documents
- 4
- Languages
- EN
- Last added
- 2012-07-02
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Agilent 6520 QTOF LC/MS System Metabolite Identification Application Note
This document details the identification of pharmaceutical metabolites during drug discovery and development. It highlights the benefits of using the Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS system combined with Agilent MassHunter Metabolite Identification (MetID) software.
Read onlineAgilent 6520 Q-TOF specifications
| System Model | Agilent 6520 Accurate-Mass O-TOF LC/MS system |
|---|---|
| Software | Agilent MassHunter Metabolite Identification software |
| Column | ZORBAX SB-C18 column, 2.1 x 150 mm, 1.8 pm |
| Flow rate | 0.5 mL/min |
| Mass range | 100-1000 |
| Collision energy | 35 V |
Source: Agilent 6520 QTOF LC/MS System Metabolite Identification Application Note
All Agilent 6520 Q-TOF documents by type
How to use the product for one specific task, with worked examples.
- Agilent 6520 QTOF LC/MS System Metabolite Identification Application Note 8 p
- Agilent Lipidomics Discovery Profiling Targeted LC/MS Analysis in 3T3-L1 Differentiating Adipocytes Application Note 8 p
- Agilent Analyzing Compounds of Environmental Interest Using an LC/Q-TOF Part 3: Imidacloprid and Manool Application Note 6 p
- Agilent 1260 Infinity and 6520 Q-TOF LC/MS Application Note: Identification of Oxidation Sites on a Monoclonal Antibody 8 p
Questions answered in these manuals
What is the purpose of this application note?
It describes the identification of pharmaceutical metabolites using the Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS system and Agilent MassHunter Metabolite Identification (MetID) software.
From: Agilent 6520 QTOF LC/MS System Metabolite Identification Application Note
How is high mass resolution utilized?
High mass resolution is used to elucidate the structures of metabolites by differentiating between fragments of isobaric mass in MS/MS spectra.
From: Agilent 6520 QTOF LC/MS System Metabolite Identification Application Note
What is the duration of the 3T3-L1 cell differentiation process described in this study?
The differentiation process is an 8-day procedure that begins with adding a hormone cocktail to pre-adipocytes.
What are the dimensions of the Gemini C18 reversed phase column used for negative ion mode runs?
The column used for negative ion mode runs has dimensions of 4.6 x 50 mm.
What is the mass accuracy for a 10 ppb imidacloprid standard?
The mass accuracy was 1.76 ppm.
What is the flow rate used during sample analysis?
The flow rate was set to 0.5 mL/min.