Key facts
- Brand
- Agilent
- Model
- 6410 triple quadrupole LC
- Documents
- 4
- Languages
- EN
- Last added
- 2012-07-02
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Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4
This document describes the experimental details and results of a plasma protein binding (PPB) assay designed for early in vitro ADME profiling. The study utilizes Agilent's rapid resolution liquid chromatography (RRLC) and triple quadrupole mass spectrometry (LC/MS/MS) systems, specifically the 1200 Series RRLC, 6410 triple quadrupole LC/MS, CTC HTC-PAL autosampler, and ZORBAX RRHT column.
The note outlines the equilibrium dialysis protocol, including sample preparation, incubation conditions, and the specific MRM method setup for various test compounds. It presents a summary of results showing the fraction unbound and recovery percentages for several compounds in human plasma.
Agilent 6410 triple quadrupole LC specifications
| Test compound summary concentration | 5 uM |
|---|---|
| Final DMSO concentration | 0.5 % |
| Final ISTD concentration | 0.18 pM |
| Plasma type | Human |
| Phosphate buffer concentration | 0.1 M |
| Incubation temperature | 37°C |
| Incubation time | overnight |
| Final Volume | 130 pl |
| Centrifugation temperature | 4 °C |
Source: Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4
All Agilent 6410 triple quadrupole LC documents by type
How to use the product for one specific task, with worked examples.
- Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4 4 p
- Agilent Improving Productivity in Early In Vitro ADME Part 3 Caco-2 Permeability Assay Application Note 4 p
- Agilent 6410 Triple Quadrupole LC/MS System Application Note: Quantification of Lovastatin in Human Plasma 8 p
Questions answered in these manuals
What equipment is used in the PPB assay described?
The study uses Agilent Equipment: 1200 Series RRLC, 6410 triple quadrupole LC/MS, CTC HTC-PAL autosampler, ZORBAX RRHT column, and MassHunter workstation software.
From: Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4
What was the incubation temperature for the plasma protein binding assay?
The incubation temperature was set to 37°C.
From: Agilent Improving Productivity in the Determination of Parameters for Early In Vitro ADME Part 4
What is the final concentration of test compounds in the Caco-2 permeability assay?
The final concentration for the test compound is 10 uM.
What is the maximum allowed DMSO concentration in the assay?
The final DMSO concentration must be 1.0%.
What is the concentration range for lovastatin quantification?
The method covers a wide range of 0.1 ppb to 50 ppb of lovastatin in human plasma.
What is the runtime for the analysis?
The method achieves a short runtime of approximately 1.2 minutes.