Key facts
- Brand
- Agilent
- Model
- 6410B
- Category
- Other Electronics
- Documents
- 3
- Languages
- EN
- Last added
- 2012-07-02
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Agilent 6410B Triple Quadrupole LC/MS Application Note
This document details the use of the Agilent 6410B Triple Quadrupole LC/MS system for detecting and identifying pharmaceutical drug metabolites. It explains the operation of the triple quadrupole instrument, which consists of three quadrupoles where Q1 and Q3 act as mass filters and Q2 acts as a collision cell for fragmentation.
The note covers specific scan modes including full scan, selected ion monitoring (SIM), product ion scan, precursor ion scan, neutral loss scan, and multiple reaction monitoring (MRM). It provides experimental details for analyzing the parent drug buspirone and its metabolites using the Agilent 1200 Series Rapid Resolution LC system and MassHunter Workstation software.
Agilent 6410B specifications
| Column dimensions | 2.1 x 150 mm |
|---|---|
| Particle size | 1.8 um |
| Flow rate | 0.5 mL/min |
| Column oven temperature | 50 C |
| Source mode | Electrospray (ESI) in positive mode |
| Dry gas flow | 12.0 L/min |
| Dry temperature | 350 C |
| Capillary voltage | 3000 V |
| Fragmentor voltage | 180 V |
| Skimmer voltage | 60V |
| Collision energy | 30 eV |
| Mass range | 50-1000 |
Source: Agilent 6410B Triple Quadrupole LC/MS Application Note
All Agilent 6410B documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the function of the middle quadrupole (Q2)?
The quadrupole in the middle (Q2) acts as a collision cell for molecular fragmentation and generation of MS/MS information.
From: Agilent 6410B Triple Quadrupole LC/MS Application Note
Which software is used for data acquisition and analysis?
The Agilent MassHunter Workstation software is used for data acquisition and qualitative data analysis.
From: Agilent 6410B Triple Quadrupole LC/MS Application Note
What is the run time for this analysis method?
The developed method has a short run time of two minutes.
How much plasma sample volume is required for preparation?
Fluvastatin and the internal standard were spiked into 250 µL of plasma.
What is the quantification range for Impurity D using the UV method?
The UV method can quantify the impurity in the range of 2.0% to 0.03%.
From: Agilent 1200 Series RRLC and 6410B LC/MS Application Note for Impurity D Quantification
What is the limit of detection for Impurity D using the ESI/MS/MS method?
The limit of detection for Impurity D using the ESI/MS/MS method is 3 ppb.
From: Agilent 1200 Series RRLC and 6410B LC/MS Application Note for Impurity D Quantification