This application note demonstrates the configuration of the Agilent 1200 Series RRLC AS purification system and the use of an optimized method for purifying dye-labeled or Fmoc-protected peptide nucleic acids from solid-phase synthesis. It provides an overview of the characteristics and applications of peptide nucleic acids.
This document describes an optimized HPLC method using the Agilent 1200 Series RRLC AS purification system to separate full-length peptide nucleic acids (PNAs) from truncated sequences and byproducts. The note details specific LC methods for both Fmoc-protected PNAs and dye-labeled PNAs, utilizing Agilent ZORBAX Eclipse columns.
It demonstrates how to configure and use the Agilent 1200 Series RRLC AS purification system for peptide nucleic acids.
The flow rate is set to 2 mL/min.