Key facts
- Brand
- Agilent
- Model
- Poroshell 300
- Category
- Other Electronics
- Documents
- 11
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
The document describes the Agilent Technologies POROSHELL 300SB-C18, a unique microparticulate superficially-porous C18 column packing designed for ultra-fast gradient separations of macromolecules. The material features a special silica support with covalently bonded sterically-protected C18 stationary phase, making it stable at low pH and suitable for aggressive mobile phases.
Read onlineAgilent Poroshell 300 specifications
| Particle Size | 5-um silica particles |
|---|---|
| Shell Thickness | 0.25-1m-thick shell of 300A pores |
| Nitrogen Surface Area | about 4.5 m?/g |
| Maximum Operating Pressure | 400 bar (6000 psi) |
| Max Temp at pH <5 | 70°C |
| Max Temp at pH 5-8 | 40° C |
Source: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
All Agilent Poroshell 300 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
- Agilent Poroshell 300SB-C18 RP-HPLC Column Product Leaflet 6 p
- Agilent Poroshell 300SB-C18 Column Product Leaflet 6 p
- Agilent ZORBAX Poroshell 300Extend-C18 High-pH Separation Application 2 p
- Agilent ZORBAX Poroshell 300Extend-C18 LC/MS Application Note 2 p
- Agilent Technologies Rapid HPLC Analysis of Monoclonal Antibody IgG1 Light Chains Using ZORBAX Poroshell 300SB-C8. 2 p
- Agilent Poroshell 300 Fast Protein Separations Technical Note 5 p
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the maximum operating pressure for this column?
The maximum operating pressure is 400 bar or 6000 psi.
From: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
What are the pH limits for using this column?
The column should be avoided below pH 0.9 or above pH 8.0.
From: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
What is the recommended maximum operating temperature for pH < 5?
The maximum operating temperature is 70 °C.
Which Agilent systems are recommended for UHPLC separations?
The Agilent 1260 Infinity, 1260 Infinity Bio-inert, or 1290 Infinity LC systems are recommended.
What is the particle size of the Poroshell 300SB-C18 column?
The column uses a 5 micron particle.
From: Agilent Poroshell 300SB-C18 RP-HPLC Column Product Leaflet
At what temperature can the column be used for separations?
The column allows separation at temperatures up to 90°C.
From: Agilent Poroshell 300SB-C18 RP-HPLC Column Product Leaflet