This application note describes the use of the Agilent 1200 Series Rapid Resolution LC (RRLC) system for the separation of antioxidants and erucamide. The system can operate significantly faster than conventional HPLC systems without sacrificing resolution, precision, or sensitivity. The column chemistry and temperature influence on the separation and the sample preparation method are also discussed.
This document details an application for analyzing additives in polymer formulations, specifically phenolic antioxidants and erucamide slip additives. It demonstrates the use of the Agilent 1200 Series Rapid Resolution LC (RRLC) system to separate these components significantly faster than conventional HPLC methods without sacrificing resolution or sensitivity.
The text outlines specific system configurations, including the G1379B microvacuum degasser, G1312B binary pump, G1367B autosampler, G1316B thermostatted column compartment, and G1315C UV/VIS diode array detector. It discusses method optimization using the ZORBAX Eclipse XDB-C18 and ZORBAX SB-C18 columns, mobile phase gradients, and temperature effects on separation speed.
| System Components | G1379B microvacuum degasser, G1312B binary pump, G1367B autosampler, G1316B thermostatted column compartment, G1315C UV/VIS diode array detector |
|---|---|
| Column Chemistry | ZORBAX Eclipse XDB-C18, ZORBAX SB-C18 |
| Mobile Phase | A: water, B: acetonitrile (ACN) |
| Analysis Time (Speed-optimized) | 1.7 minutes |
| Maximum Column Temperature | 90 °C |
The application can achieve comparable results in as few as 3 minutes, which is significantly faster than conventional HPLC methods that often require more than 30 minutes.
The method can determine 1 ppm of additives with very good signal-to-noise response, which exceeds the ASTM specification of 2 ppm.