Key facts
Start here
Agilent 7000 Triple Quadrupole GC/MS Estradiol Detection Application Brief
This document details a method developed on the Agilent 7890GC/7000 Triple Quadrupole GC/MS system to detect and quantify estradiol in human plasma. The method achieves a limit of detection (LOD) of 1 pg/mL (2 fg on-column) and maintains a relative standard deviation (RSD) for quantification of less than 15% across a concentration range of 1 to 600 pg/mL. The analysis utilizes the MS/MS-NCI (negative chemical ionization) mode with a specific column and injection sequence.
Read onlineAgilent 7000 Triple Quadrupole GC specifications
| Limit of Detection (LOD) | 1 pg/mL (2 fg on-column) |
|---|---|
| Quantification Range | 1 to 600 pg/mL |
| Relative Standard Deviation (RSD) | <15% |
| Analytical Column | 15m x 0.25 mm x 0.25 pm Agilent J&W DB-5MS UI |
| Carrier Gas | Helium, constant flow, 1.2 mL/min |
| MS Mode | NCI mode; 40% ammonia |
| Source Temperature | 150 °C |
| Quadrupoles Temperature | 150 °C |
| Collision Gas Flows | Nitrogen at 1.5 mL/min, Helium at 2.35 mL/min |
| Total Run Time | 2.85 minutes |
Source: Agilent 7000 Triple Quadrupole GC/MS Estradiol Detection Application Brief
All Agilent 7000 Triple Quadrupole GC documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the limit of detection for estradiol using this method?
The limit of detection is 1 pg/mL, which corresponds to 2 fg injected on-column.
From: Agilent 7000 Triple Quadrupole GC/MS Estradiol Detection Application Brief
What is the quantification range for estradiol?
The method provides quantification for estradiol in the range of 1 to 600 pg/mL.
From: Agilent 7000 Triple Quadrupole GC/MS Estradiol Detection Application Brief
What instrument is used for this analysis?
The analysis is performed on an Agilent 7890 GC coupled with an Agilent 7000 Triple Quadrupole GC/MS system.
From: Agilent 7000 Triple Quadrupole GC/MS PCB Analysis Application Note
What is the detection limit for dioxin-like PCB congeners in this method?
The method provides quantitative results for dioxin-like PCB congeners down to low pg TEQ/g levels.
From: Agilent 7000 Triple Quadrupole GC/MS PCB Analysis Application Note