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Agilent Human 14 Multiple Affinity Removal Spin Cartridge Instruction Guide
This document provides step-by-step instructions for using the Agilent Human 14 Multiple Affinity Removal Spin Cartridge to process human serum or plasma samples. It details procedures for dilution, filtration, sample application, centrifugation, elution, and re-equilibration. The guide also includes information on storing the cartridge and analyzing collected fractions.
The document references additional Agilent products for proteomics research, such as LC columns, digestion kits, and cleanup tools, and directs users to Agilent’s website for more information.
Agilent Human 14 specifications
| Average Column Capacity | 40 uL of plasma or serum |
|---|---|
| Total Protein Depletion | Approximately 94% |
| Column Lifetime | Over 200 runs |
Source: Agilent Human 14 Multiple Affinity Removal System Application Note
All Agilent Human 14 documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
Which proteins does the Human 14 column deplete?
It depletes 14 high-abundant proteins including HSA, transferrin, haptoglobin, IgG, IgA, alpha-1-antitrypsin, fibrinogen, alpha-2-macroglobulin, alpha-acid glycoprotein, complement C3, IgM, apolipoprotein AI, apolipoprotein AII, and transthyretin.
From: Agilent Human 14 Multiple Affinity Removal System Application Note
How does the Human 14 column compare to the Human 6 column?
The Human 14 column removes approximately 10% greater total protein content than the Human 6 column.
From: Agilent Human 14 Multiple Affinity Removal System Application Note
How do I prepare the sample for processing?
Dilute 8-10 pL human serum/plasma to 200 μL with Buffer A, then filter through a 0.22 μm spin filter.
From: Agilent Human 14 Multiple Affinity Removal Spin Cartridge Instruction Guide
What is the part number for the Agilent Human 14 Multiple Affinity Removal Spin Cartridge?
The part number is 5188-8560.
From: Agilent Human 14 Multiple Affinity Removal Spin Cartridge Instruction Guide