Key facts
- Brand
- Agilent
- Model
- 1100 LC
- Documents
- 9
- Languages
- EN
- Last added
- 2012-07-05
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Metabolite Identification Using ACD Labs Version 8.0 Software and the Agilent 1100 LC/MSD Trap XCT Plus System Applicati
This document details the use of ACD Labs MS Manager Suite Version 8.0 in conjunction with an Agilent 1100 Series LC/MSD Trap XCT Plus ion trap mass spectrometer for metabolite identification studies.
The text outlines a workflow involving data import from multiple instruments, automatic comparison of control and sample data using the CODA algorithm, and structural analysis via ChemSketch. It also covers in-silico fragmentation modeling to assign structural features to mass spectral peaks.
Agilent 1100 LC specifications
| Column | ZORBAX SB-C18, 4.6 mm x 30 mm x 3.5 pm |
|---|---|
| Mobile phase | Methanol: 50-mM ammonium acetate in water (60:40) |
| Flow rate | 1 mL/min |
| Injection Volume | 2.5 pL |
| Nebulizer pressure and temperature | 25 psi at 350 °C |
| Drying gas flow rate | 9 L/min |
Source: Agilent 1100 LC/MSD Therapeutic Drug Monitoring Application Note: Clozapine Example
All Agilent 1100 LC documents by type
How to use the product for one specific task, with worked examples.
- Metabolite Identification Using ACD Labs Version 8.0 Software and the Agilent 1100 LC/MSD Trap XCT Plus System Applicati 10 p
- Agilent 1100 LC/MSD Therapeutic Drug Monitoring Application Note: Clozapine Example 8 p
- Agilent 1100 LC/MSD Performance-Based Mass Spectral Library Application Note 14 p
- Agilent Determination of LSD iso-LSD 2-Oxo-3-Hydroxy-LSD in Urine Using the HP1100 LC/MSD 4 p
- Agilent 1100 LC/MS Analysis of Simazine Thiobencarb Thiuram 2 p
- Agilent 1100 LC/MSD Application Note: Identification and Isolation of DSP-Toxins 8 p
- Chloramphenicol Detection in Honey and Shrimp Using Agilent 1100 LC/MSD Trap Application Note 10 p
- Agilent 1100 LC/MS Analysis of Triglycerides Application Note 4 p
Questions answered in these manuals
What software modules are included in the ACD Labs MS Manager Suite?
The suite includes MS Manager, ChroManager, UVIR Manager, MS Fragmenter, ChemSketch, SpecDB, ChemFolder, ChemBasic, HNMR Viewer, and CNMR Viewer.
Which instrument was used to acquire the data in this note?
The data was acquired using an Agilent LC/MS system consisting of an Agilent 1100 Series solvent degasser, binary pump, well plate sampler, thermostatted column compartment, and an Agilent 1100 Series LC/MSD XCT Plus ion trap mass spectrometer.
What is the duration of this training course?
The course lasts for four days.
From: Agilent 1100 LC/MSD Ion Trap Techniques and Software Operation Course Handbook
Which operating systems are required to run the equipment used in training?
Computers must have Microsoft Windows NT or 2000 installed.
From: Agilent 1100 LC/MSD Ion Trap Techniques and Software Operation Course Handbook
What instrument is used for the clozapine analysis?
The analysis is performed using the Agilent Technologies 1100 LC/MSD.
From: Agilent 1100 LC/MSD Therapeutic Drug Monitoring Application Note: Clozapine Example
What are the typical therapeutic serum levels for clozapine patients?
Typical serum levels for patients range from 450 nM to 3000 nM.
From: Agilent 1100 LC/MSD Therapeutic Drug Monitoring Application Note: Clozapine Example