Key facts
- Brand
- Agilent
- Model
- P450
- Industry
- Industrial
- Documents
- 3
- Languages
- EN
- Last added
- 2012-07-02
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Cytochrome P450 Assay on the Agilent Bravo Platform Application Bulletin
Cytochrome P450 enzymes are critical in drug discovery, and this document outlines a high-throughput assay protocol. The method utilizes the Promega P450-Glo kit with the Agilent Bravo Automated Liquid Handling Platform to measure enzyme activity via a luminescent signal.
The protocol details a two-step reaction process involving substrate metabolism and detection reagent addition. It specifies the required materials, including the Bravo Platform with specific gripper and pipette head configurations, as well as the necessary labware and reagents for processing up to five microplates simultaneously.
Agilent P450 specifications
| Run time per sample | approximately 6 s |
|---|---|
| Comparison run time | 2-4 min |
| Throughput increase | greater than 10-fold |
| Productivity increase | approximately 20-fold |
Source: Agilent RapidFire/MS Cytochrome P450 Inhibition Application Note
All Agilent P450 documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
How long does it take to process five microplates?
The microplate processing time is approximately one hour for a run of five plates.
From: Cytochrome P450 Assay on the Agilent Bravo Platform Application Bulletin
What volume of P450/Substrate mix should be dispensed?
You must dispense 6.25 pL of the P450/Substrate mixture from reservoir A.
From: Cytochrome P450 Assay on the Agilent Bravo Platform Application Bulletin
What are the FDA recommended drug probes for CYP3A4/5?
The FDA recommended drug probes for CYP3A4/5 are midazolam, testosterone, nifedipine, and erythromycin.
From: Agilent RapidFire/MS Cytochrome P450 Inhibition Application Note
Does the RapidFire system produce equivalent results to LC/MS/MS?
Yes, the RapidFire system produces equivalent results to LC/MS/MS for CYP inhibition assays.
From: Agilent RapidFire/MS Cytochrome P450 Inhibition Application Note
What are the FDA recommended drug probes for CYP3A4?
The FDA preferred substrates for CYP3A4 are midazolam and testosterone.
Does the RapidFire system produce equivalent results to LC/MS/MS?
Yes, correlation of IC50 values indicates that the RapidFire system produces equivalent results to traditional LC/MS/MS.