Key facts
- Brand
- Agilent
- Model
- PREP-C18
- Industry
- Industrial
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Prep-C18 Columns Datasheet
This document provides a technical overview of the Agilent Prep-C18 columns, which utilize ultra-pure silica support with high specific surface area and 100A pore size. Available in two particle sizes (5-microns and 10-microns) and various internal diameters, these columns are designed for the preparative purification of acidic, basic, and highly polar compounds via reversed-phase liquid chromatography.
Read onlineAgilent PREP-C18 specifications
| Particle Size | 5-microns or 10-microns |
|---|---|
| Carbon Loading | 24% |
| pH Stability Range | 2-10 |
| Maximum Operating Pressure | 340 bar (5000 psi) |
| Maximum Operating Temperature | 60°C |
All Agilent PREP-C18 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the recommended storage solvent for long-term storage?
Long term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
What is the maximum operating pressure for these columns?
The maximum operating pressure limit is 340 bar or 5000 psi.
What are the three calcium channel blockers separated in this study?
The study separates Nifedipine, Nimodipine, and Nisoldipine.
From: Agilent Prep-C18 Preparative Scaleup of Calcium Channel Blockers Application Note
What is the recommended resolution threshold for stopping the scaleup process?
The scaleup process should stop when resolution drops below 1.2.
From: Agilent Prep-C18 Preparative Scaleup of Calcium Channel Blockers Application Note
What is the maximum loadability of the 30-mm Agilent Prep C18 column?
The column can handle up to 320 mg of total corticosteroid before overloading begins.
What solvent is recommended for injection in preparative chromatography?
Dimethylsulfoxide (DMSO) is recommended because it dissolves organic compounds well and elutes rapidly.