Key facts
- Brand
- Agilent
- Model
- GF-450
- Industry
- Industrial
- Documents
- 2
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Zorbax PrepHT GF-250 and GF-450 Datasheet
The document describes the Agilent Zorbax PrepHT GF-250 and GF-450 as surface-stabilized, hydrophilic gel filtration columns designed for the size separation of biological macromolecules. These columns offer superior efficiency and pH stability when used with aqueous buffer solutions in the pH range of 3.0 to 8.5.
Read onlineAgilent GF-450 specifications
| Optimal Separation Range (GF-450) | 900,000 to 25,000 Daltons |
|---|---|
| Optimal Separation Range (GF-250) | 250,000 to 10,000 D |
| Recommended pH Limits | pH 3 to 8.5 |
| Maximum Operating Temperature | 40°C |
| Protein Recovery | 85-100% |
All Agilent GF-450 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the recommended pH range for these columns?
The recommended pH limits for these columns are pH 3 to 8.5.
Can the columns be used with organic solvents?
Yes, the GF-250 and GF-450 columns are compatible with most organic solvents.
What molecular weight range can the ZORBAX GF-450 column separate?
The column separates biomolecules within a molecular-weight range of 10,000 to 1,000,000 daltons.
What are the dimensions and part number for the ZORBAX GF-450 column used in this test?
The column has dimensions of 9.4 x 250 and Agilent Part No. 884973-902.