Key facts
- Brand
- Agilent
- Model
- MRP-C18
- Industry
- Industrial
- Documents
- 4
- Languages
- EN
- Last added
- 2012-07-04
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Agilent mRP-C18 Macroporous Reversed-Phase C18 Column Datasheet
The Agilent mRP-C18 column is a unique silica-based C18 packing designed specifically for the separation of complex protein mixtures. It features high protein recoveries, consistent reproducibility, and enhanced resolution compared to conventional silica-based RP HPLC columns. The column utilizes ultrapure, less acidic 5-um silica particles packed in PEEK hardware to minimize protein adsorption.
This document provides operational guidelines, safety considerations, and recommended applications for the mRP-C18 column. It details sample preparation methods for protein fractionation and desalting, including specific gradient conditions and temperature settings. The datasheet also includes ordering information for the 4.6 mm id x 50 mm column.
Agilent MRP-C18 specifications
| Particle Size | 5-um |
|---|---|
| Maximum Protein Loading | 380 ug |
| Maximum Operating Pressure | 250 bar (4000 psi) |
| Maximum Operating Temperature (pH <5) | 80 °C |
| Maximum Operating Temperature (pH 5-8) | 40 °C |
| Recommended pH Range | pH 0.9 to 8.0 |
Source: Agilent mRP-C18 Macroporous Reversed-Phase C18 Column Datasheet
All Agilent MRP-C18 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the recommended pH range for the mRP-C18 column?
The column should be used between pH 0.9 and 8.0, as it is designed for high stability at low pH (e.g., pH <5).
From: Agilent mRP-C18 Macroporous Reversed-Phase C18 Column Datasheet
What is the maximum protein loading capacity for a 4.6 x 50 mm mRP-C18 column?
The maximum protein loading is 380 ug.
From: Agilent mRP-C18 Macroporous Reversed-Phase C18 Column Datasheet
What protein recovery rate does the mRP-C18 column offer?
The column offers greater than 95 percent protein recovery, with observed recoveries of greater than 98 percent for immunodepleted serum.
From: Agilent mRP-C18 High-Recovery Protein Fractionation HPLC Column Product Leaflet
How long does desalting take when using this column?
The column allows you to desalt in about 13 minutes, eliminating the need for a normally three-hour spin concentration process.
From: Agilent mRP-C18 High-Recovery Protein Fractionation HPLC Column Product Leaflet
How many proteins were identified using the described method?
The method identified more than 954 proteins, including 470 membrane proteins and 337 integral membrane proteins.
From: Agilent High Recovery mRP-C18 Column Proteomic Strategy Application Note
What specific cell line was used to demonstrate this proteomic strategy?
The strategy was demonstrated using HeLa cell membrane proteins.
From: Agilent High Recovery mRP-C18 Column Proteomic Strategy Application Note