Agilent EC-C18 Manuals

The paper introduces the characteristics of superficially porous particles and discusses the transfer of methods from larger particle columns (5-µm) to superficially porous particle columns (120 EC-C18) and sub-2-µm totally porous columns (ZORBAX Eclipse Plus C18)

This document introduces a fast method for analyzing environmental phenols by using Agilent Poroshell 120 EC-C18 4.6 × 100 mm, 2.7 µm column, which transfers the method originally developed on a 4.6 × 100 mm, 5-µm column. The gradient and flow rate are scaled while maintaining a constant retention index in order to determine the optimal flow rate for each evaluated column. Simple guidelines for transferring a method are provided. The new separation reduces the analysis time from 20 minutes to less than 3 minutes with a higher peak capacity, and should not require any changes to sample preparation since both columns use 2 µm frits. The method is further scaled to a 50 mm column that produces slightly less resolution than found with the 100 mm column, but with an analysis time of only 2 minutes with an equivalent peak capacity to the original method. While pressure is increased from the original method, it is still below 400 bar and can be easily transferred to any HPLC system.