Key facts
- Brand
- Agilent
- Model
- ZORBAX 300SB-C18
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-05
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Agilent ZORBAX RRHD 300SB-C18 Technical Overview
The document provides a technical overview of the Agilent ZORBAX RRHD 300SB-C18 1.8 µm column, designed for ultra-high-performance liquid chromatography (UHPLC) of proteins and peptides. It highlights the use of StableBond technology to ensure stability under acidic conditions required for protein analysis.
The text details application examples using short 50 mm columns for intact protein separation at various flow rates and temperatures, as well as longer 100 mm columns for peptide digest analysis. Reproducibility data after multiple runs is also presented.
Agilent ZORBAX 300SB-C18 specifications
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Column Part Numbers | 883995-902, 883995-906, 883995-905 |
| Mobile Phase A | 0.1% TFA in H2O |
| Mobile Phase B | 0.085% TFA in ACN |
| Flow Rate | 1 mL/min |
| Detection Wavelength | 210 nm |
| Gradient Program | 0-30% B in 30 min |
Source: Agilent ZORBAX 300SB Series Effect of Bonded Phase Type on Peptide Separation
All Agilent ZORBAX 300SB-C18 documents by type
How to use the product for one specific task, with worked examples.
Ratings, dimensions and ordering codes, for selection and design work.
Questions answered in these manuals
What is the stated purpose of the Agilent ZORBAX RRHD 300SB-C18 1.8 pm column?
It is a reverse phase media designed for UHPLC analysis of proteins and peptides.
What are the dimensions of the column used for analyzing intact proteins?
The column used for intact protein analysis has dimensions of 2.1 x 50 mm.
What is the effect of changing the bonded phase on peptide separation?
Significant differences in selectivity can be achieved by changing the ZORBAX StableBond phase.
From: Agilent ZORBAX 300SB Series Effect of Bonded Phase Type on Peptide Separation
What are the part numbers for the ZORBAX columns tested?
The part numbers are 883995-902, 883995-906, and 883995-905.
From: Agilent ZORBAX 300SB Series Effect of Bonded Phase Type on Peptide Separation
What is the purpose of this application note?
This document describes the separation of peptide fragments generated by treating recombinant human growth hormone with trypsin.
From: Agilent ZORBAX 300SB-C18 Separation of rhGH Tryptic Digest Fragments Application Note
What are the dimensions of the column used in this separation?
The procedure uses a column that is 1 mm in internal diameter and 150 mm in length.
From: Agilent ZORBAX 300SB-C18 Separation of rhGH Tryptic Digest Fragments Application Note