The Macroporous Reversed-Phase C18 Column (mRP-C18) is a unique silica-based C18 column packing produced by Agilent. It has high protein recoveries, consistent reproducibility, increased column loads, and enhanced resolution compared to conventional silica-based RP HPLC columns.
The Agilent mRP-C18 column is a unique silica-based C18 packing designed specifically for the separation of complex protein mixtures. It features high protein recoveries, consistent reproducibility, and enhanced resolution compared to conventional silica-based RP HPLC columns. The column utilizes ultrapure, less acidic 5-um silica particles packed in PEEK hardware to minimize protein adsorption.
This document provides operational guidelines, safety considerations, and recommended applications for the mRP-C18 column. It details sample preparation methods for protein fractionation and desalting, including specific gradient conditions and temperature settings. The datasheet also includes ordering information for the 4.6 mm id x 50 mm column.
| Particle Size | 5-um |
|---|---|
| Maximum Protein Loading | 380 ug |
| Maximum Operating Pressure | 250 bar (4000 psi) |
| Maximum Operating Temperature (pH <5) | 80 °C |
| Maximum Operating Temperature (pH 5-8) | 40 °C |
| Recommended pH Range | pH 0.9 to 8.0 |
The column should be used between pH 0.9 and 8.0, as it is designed for high stability at low pH (e.g., pH <5).
The maximum protein loading is 380 ug.
Long term storage should be in a pure organic solvent, preferably 100% acetonitrile.