Key facts
- Brand
- Agilent
- Model
- ZORBAX GF-250
- Documents
- 8
- Languages
- EN
- Last added
- 2012-07-03
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Agilent ZORBAX GF-250 Application Note: Comparison of Denaturing and non-Denaturing Mobile Phases
This document is an application brief by Robert D. Ricker from Agilent Technologies regarding size-exclusion chromatography (SEC). It discusses the use of standard non-denaturing mobile phases versus denaturing mobile phases containing guanidine hydrochloride (GuCl) or SDS. The text explains that denaturing phases are used to ensure un-aggregated monomers for proper size determination, noting that this shifts molecules toward larger apparent sizes and smaller retention volumes.
Read onlineAgilent ZORBAX GF-250 specifications
| Molecular-weight range | 4,000-400,000 daltons |
|---|---|
| Column dimensions | 9.4 x 250 mm |
| Part Number | 884973-901 |
| Detection wavelength | 254 nm |
| Flow rate | 2.0 mL/min |
| Injection volume | 20 uL |
Source: Agilent ZORBAX GF-250 Application Note: Comparison of Denaturing and non-Denaturing Mobile Phases
All Agilent ZORBAX GF-250 documents by type
How to use the product for one specific task, with worked examples.
- Agilent ZORBAX GF-250 Separation of Bio-Rad Gel-Filtration Standards Application Note 2 p
- Agilent ZORBAX GF-250 Column Calibration Using Bio-Rad Gel-Filtration Standards Application Note 2 p
- Agilent Comparison of Denaturing non-Denaturing Mobile Phases in ZORBAX GF-2501 2 p
- Agilent ZORBAX GF-250 SEC Application Note 2 p
- Agilent ZORBAX GF-250 Column Calibration with Globular Proteins Application Note 2 p
Questions answered in these manuals
What is the purpose of using denaturing mobile phases in SEC?
Denaturing mobile phases containing guanidine hydrochloride or SDS are used to insure un-aggregated, monomers for proper size determination.
From: Agilent ZORBAX GF-250 Application Note: Comparison of Denaturing and non-Denaturing Mobile Phases
How does denaturation affect the separation results?
Denaturation shifts molecules toward larger apparent size and smaller retention volume.
From: Agilent ZORBAX GF-250 Application Note: Comparison of Denaturing and non-Denaturing Mobile Phases
What is the stated column dimension for the ZORBAX GF-250 in this procedure?
The application uses a ZORBAX GF-250 column with dimensions of 4.6 x 250 mm.
From: Agilent ZORBAX GF-250 Fast Separation of IgM and IgG Antibodies Application Note
What is the stated flow rate for the separation of IgM and IgG antibodies?
The procedure specifies a flow rate of 0.94 mL/min for the separation.
From: Agilent ZORBAX GF-250 Fast Separation of IgM and IgG Antibodies Application Note
What is the stated column size for the ZORBAX GF-250?
The document specifies a column size of 9.4 x 250 mm.
From: Agilent ZORBAX GF-250 Separation of Bio-Rad Gel-Filtration Standards Application Note
What is the recommended mobile phase for this separation?
Use 200mM Sodium Phosphate at pH 7.0 as the mobile phase.
From: Agilent ZORBAX GF-250 Separation of Bio-Rad Gel-Filtration Standards Application Note