Key facts
- Brand
- Agilent
- Model
- MX3000P
- Industry
- Industrial
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-04
Start here
Agilent Mx3000P/Mx3005P QPCR System Instrument Qualification Beta-Actin Install Plate Instructions
This document provides instructions for the storage, handling, and preparation of the Beta-Actin Install Plate used with the Agilent Mx3000P or Mx3005P QPCR System. It details procedures such as thawing, mixing by inversion, centrifugation, and bubble removal to ensure accurate qualification testing.
Read onlineAgilent MX3000P specifications
| Reaction Time | under 50 minutes |
|---|---|
| Compatibility | Mx3000P and Mx3005P |
| Amplification Efficiency | 96.4% |
| Standard Curve R2 | 0.999 |
| RT Synthesis Temperature | 50°C |
| Packaging Qty | 2 x 2 ml |
Source: Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for Mx3005P/Mx3000P handbook
All Agilent MX3000P documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the maximum reaction time for Brilliant III Ultra-Fast reagents?
The reagents allow the completion of real-time experiments in less than 50 minutes on the Mx platform.
From: Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for Mx3005P/Mx3000P handbook
Which Agilent instruments are validated for these reagents?
The reagents have been validated on the Agilent Mx3000P and Mx3005P instruments.
From: Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for Mx3005P/Mx3000P handbook
What four foodborne pathogens can be detected using these assays?
The assays are designed to detect Salmonella enterica, Escherichia coli O157:H7, Listeria monocytogenes, and Campylobacter jejuni.
How long does it take to obtain results after sample enrichment?
Results can be obtained with these assays in 2.5 h following enrichment.
What is the linear detection range of the multiplex assay for mycotoxigenic fungi?
The linear detection range of the multiplex assay is between 10 pg to 10 ng of DNA.
From: Agilent Mx3000P QPCR System Application Note for Mycotoxigenic Fungi Detection
What are the optimum parameters for primer and probe concentration in the PCR reaction?
The optimum parameters are 10 pmol of each primer and 5 pmol of probe per reaction.
From: Agilent Mx3000P QPCR System Application Note for Mycotoxigenic Fungi Detection