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Agilent Ultra-fast liquid chromatography using the Agilent 1100 Series HPLC system 1.8-um ZORBAX SB C18 Rapid Resolution HT columns Manual
This document describes the recommended system configuration to achieve very fast analysis and cycle times using ZORBAX 1.8-um Rapid Resolution High Throughput (RRHT) columns on the Agilent 1100 Series liquid chromatograph. It details how to reduce analysis time to seconds without compromising resolution or chromatographic performance.
Read onlineAgilent ZORBAX SB-C18 Rapid-Resolution specifications
| Analysis time | 29 seconds |
|---|---|
| Peak width | 0.5 seconds |
| Backpressure | 300 bar |
| Column particle size | 1.8 um |
| Delay volume | 300 pL |
| Injection speed | < 30 seconds |
| Flow cell volume | 500-nL |
All Agilent ZORBAX SB-C18 Rapid-Resolution documents by type
- Agilent Ultra-fast liquid chromatography using the Agilent 1100 Series HPLC system 1.8-um ZORBAX SB C18 Rapid Resolution HT columns Manual 12 p
- Agilent ZORBAX SB-C18 Rapid-Resolution High-Speed Separation of Analgesics Application Note 2 p
- Agilent ZORBAX SB-C18 Rapid-Resolution Column Application Note 2 p
Questions answered in these manuals
What is the minimum configuration for ultra-fast analysis?
The minimum configuration consists of the Agilent 1100 Series binary pump, well-plate autosampler, thermostatted column compartment, and ZORBAX 1.8-um RRHT columns.
What is the benefit of overlapped injections (OD)?
Overlapped injection allows the preparation of the next injection while the current injection is being completed, increasing sample throughput.
What is the analysis time for this method?
The method resolves seven analgesics in less than one minute.
From: Agilent ZORBAX SB-C18 Rapid-Resolution High-Speed Separation of Analgesics Application Note
Can this method be used for LC/MS?
Yes, the volatile buffer can be used in place of the ion-pair mobile phase for LC/MS.
From: Agilent ZORBAX SB-C18 Rapid-Resolution High-Speed Separation of Analgesics Application Note
What are the benefits of using low-volume columns?
Low-volume columns are valuable for high-speed analyses, decreasing solvent consumption, and enhancing sensitivity for mass-limited samples commonly used for LC/MS applications.
From: Agilent ZORBAX SB-C18 Rapid-Resolution Column Application Note
How much does analysis time reduce when switching to a shorter column?
Switching from a 150-mm to a 15-mm column reduces analysis time and solvent use ten-fold.
From: Agilent ZORBAX SB-C18 Rapid-Resolution Column Application Note